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银边红雀珊瑚(Pedilanthus tithymaloides(L) Poir.var.cuculatus Hort.)快速繁殖研究
引用本文:龙明华,覃海明.银边红雀珊瑚(Pedilanthus tithymaloides(L) Poir.var.cuculatus Hort.)快速繁殖研究[J].广西科学,1996,3(1):53-55.
作者姓名:龙明华  覃海明
作者单位:广西农业大学, 南宁市秀灵路13号 530005,广西农业大学, 南宁市秀灵路13号 530005
摘    要:顶芽或腋芽在添加6-苄基氨基嘌呤(6-BA)3ppm和萘乙酸(NAA)0.5ppm的MS培养基培养30d,长4-6cm,腋芽3-7个。此时,将它们分切成单芽切段,围到添加6-BA2ppm的MS培养基,继代培养周期21d,繁殖系数5-8。芽长2-3cm时用添加吲哚丁酸(IBA)1ppm的MS培养基进行生根培养,培养21d,生根率87%,移载成活率93%。

关 键 词:银边红雀珊瑚  微型扦插  快速繁殖
收稿时间:1994/9/24 0:00:00
修稿时间:1995/8/12 0:00:00

A Study on the Rapid Micropropagation of Pedilanthus tithymaloides (L.) Poir.var.cuculatus Hort
Long Minghua and Qin Haiming.A Study on the Rapid Micropropagation of Pedilanthus tithymaloides (L.) Poir.var.cuculatus Hort[J].Guangxi Sciences,1996,3(1):53-55.
Authors:Long Minghua and Qin Haiming
Institution:Guangxi Agricultural University, 13 Xiulin Road, Nanning, Guangxi, 530005 and Guangxi Agricultural University, 13 Xiulin Road, Nanning, Guangxi, 530005
Abstract:Apical buds or axillary buds were cultured on the MS medium supplemented with benzylaminopurine (6-BA) 3 mg/L and naphthalene-acetic acid (NAA) 0.5 mg/L for 30 d. They grew to be 4~6 cm in length with 3~7 axillary buds. Then, they were cut into segments with one bud,and cultured on MS medium supplemented with 6-BA 2 mg/L for subculture. Subculture cycle was 21 d and multiplication rate was 5~8. Buds were cultured on MS medium supplemented with indolebutyric acid (IBA) 1 mg/L for rooting when length of them was 2~3 cm. Rooting rate was 87% and 93% rooting plantlets were successfully transplanted to pots in the greenhouse.
Keywords:Pedilanthus tithymaloides(L  ) Poir  var  cuculatus Hort    microcutting  rapid micropropagation
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