顶空固相微萃取结合气质联用方法快速测定地椒挥发性成分

采用顶空固相微萃取法(headspace solid-phase microextraction, HS-SPME)结合气质联用技术(gas chromatography-mass spectrometry,GC-MS)提取、分析地椒中的挥发性化学成分,通过面积归一化法确定各成分的相对含量。结果检出挥发性成分56种,其中以酚醇类、萜类、烯类、酯类、醛酮类化合物为主,主要成分有芳樟醇(27.8%)、龙脑(9.12%)、樟脑萜(8.12%)、柠檬烯(7.81%)等。本实验首次对山东地区地椒挥发性成分进行HS-SPME结合GC-MS研究,发现其化学成分丰富,具有较高的药用价值。

Rapid analysis of the volatile components of Mongolian thyme herb's essential oils using headspace solid-phase microextraction combined with gas chromatography-mass spectrometry

The headspace solid-phase microextraction (HS-SPME) method combined with gas chromatography-mass spectrometry (GC-MS) was used to extract and analyze the volatile components of Mongolian thyme herb,the peak areas were normalized to obtain the relative content of each component. A total of 56 volatile components were detected, of which, phenolic alcohols, terpenes, alkenes, esters, aldehydes, and ketones were the primary chemical compounds. linalool (27.8%), borneol (9.12%), camphor terpene (8.12%), limonene (7.81%), etc. were found to be the main ingredients. This is the first study wherein HS-SPME was performed in combination with GC-MS to analyze the volatile components of Mongolian thyme herb found in the Shandong area. The chemical components were found to be rich and have a high medicinal value.