| 云芝漆酶理化性质及动力学研究 |
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| 引用本文： | 邵强,姚朝阳,郭伟云,李宗义.云芝漆酶理化性质及动力学研究[J].河南师范大学学报(自然科学版),2005,33(3):109-112. |
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| 作者姓名： | 邵强 姚朝阳 郭伟云 李宗义 |
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| 作者单位： | 河南师范大学,生命科学学院,河南,新乡,453007;河南师范大学,生命科学学院,河南,新乡,453007;河南师范大学,生命科学学院,河南,新乡,453007;河南师范大学,生命科学学院,河南,新乡,453007 |
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| 基金项目： | 河南省科技攻关项目(001200217)，河南省动物学重点学科资助项目 |
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| 摘 要： | 主要研究pH值、金属离子、温度对云芝漆酶活性和稳定性的影响,以及该酶底物浓度效应和Km值测定.实验结果表明:Cu2+、Co2+对漆酶有激活作用,而Ag+则有抑制作用;该酶最适pH为4.8,在pH4~4.8表现出较强的稳定性;最适反应温度为40℃,低于50℃时有较好的热稳定性;Km值为4.2×10-3mol/L.
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| 关 键 词： | 漆酶 pH 温度 金属离子 Km |
| 文章编号： | 1000-2367(2005)03-0109-04 |
| 收稿时间： | 2005-03-02 |
| 修稿时间： | 2005年3月2日 |
Studies on Characteristics of Laccase from Coriolus Versicolor |
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| SHAO Qiang,YAO Zhao-yang,GUO Wei-yun,LI Zong-yi.Studies on Characteristics of Laccase from Coriolus Versicolor[J].Journal of Henan Normal University(Natural Science),2005,33(3):109-112. |
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| Authors: | SHAO Qiang YAO Zhao-yang GUO Wei-yun LI Zong-yi |
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| Abstract: | The effect of pH, metal ion and temperature on Coriolus versicolor laccase activity and stability was studied in the paper. Meanwhile, the substrate concentration effect of this laccase is also studied and Km is measured. The results showed: Cu~(2+) and Co~(2+) had activation but Ag~+ had inhibition on activity of laccase. The optimum pH of the laccase was 4.8, and the laccase was stable from pH4 to 4.8. The optimum temperature of laccase was 40 ℃; and the laccase could work continuously under 50 ℃. The Km of the laccase measured 4.2×10~(-3) mol/L. |
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| Keywords: | laccase metal ion pH temperature Km |
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