| 环糊精水解酶底物运输通道的动态结构分析 |
| |
| 引用本文： | 闭海,莫莉,郭媛,林丽华,黄日波,庞浩.环糊精水解酶底物运输通道的动态结构分析[J].广西科学,2014,21(1):12-16. |
| |
| 作者姓名： | 闭海 莫莉 郭媛 林丽华 黄日波 庞浩 |
| |
| 作者单位： | 广西大学生命科学与技术学院;广西科学院;非粮生物质酶解国家重点实验室;国家非粮生物质能源工程技术研究中心;广西生物质产业化工程院;广西生物炼制重点实验室; |
| |
| 基金项目： | 广西科技合作与交流计划项目(桂科合1347004-1);广西科技攻关项目(桂科攻10123007-3);广西科学院基本科研业务费项目(12YJ25SW05,13YJ22SW);国家自然科学基金项目(21366007)资助 |
| |
| 摘 要： | 【目的】环糊精水解酶是作用于特殊底物的水解酶,可以水解圆锥形结构的底物。分析这个酶的底物通道为水解特殊结构底物提供研究基础。【方法】利用分子动力学模拟环糊精水解酶存在的底物通道,比较环糊精水解酶BsCMD_1J0H和TspCMD_1SMA在底物运输通道上的差异。【结果】BsCMD_1J0H和TspCMD_1SMA都为糖基水解酶家族13的蛋白质,它们的碳骨架基本吻合,而在α?螺旋分布上,TspCMD_1SMA相比于BsCMD_1J0H来说,螺旋结构更趋向于在蛋白质中心聚集。BsCMD_1J0H有6条底物通道连通蛋白质表面和活性中心,TspCMD_1SMA有8条底物通道连通。BsCMD_1J0H的底物通道平均半径为1,TspCMD_1SMA的底物通道平均半径为1.2。【结论】本研究提供了BsCMD_1J0H和TspCMD_1SMA两个蛋白质在和底物接触中的相关底物通道信息。
|
| 关 键 词： | 环糊精水解酶 底物通道 D结构 底物运输 淀粉 |
| 收稿时间： | 2013/8/13 0:00:00 |
| 修稿时间： | 2013/11/4 0:00:00 |
Dynamic Structural Study on Substrate Transport Channels of Cyclodextrin Hydrolase |
| |
| BI Hai,MO Li,GUO Yuan,LIN Li-hu,HUANG Ri-bo and PANG Hao.Dynamic Structural Study on Substrate Transport Channels of Cyclodextrin Hydrolase[J].Guangxi Sciences,2014,21(1):12-16. |
| |
| Authors: | BI Hai MO Li GUO Yuan LIN Li-hu HUANG Ri-bo and PANG Hao |
| |
| Institution: | College of Life Science & Technology of Guangxi University, Nanning, Guangxi, 530004, China,College of Life Science & Technology of Guangxi University, Nanning, Guangxi, 530004, China,Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China,College of Life Science & Technology of Guangxi University, Nanning, Guangxi, 530004, China;Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China,College of Life Science & Technology of Guangxi University, Nanning, Guangxi, 530004, China;Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China and College of Life Science & Technology of Guangxi University, Nanning, Guangxi, 530004, China;Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China |
| |
| Abstract: | |
| |
| Keywords: | cyclodextrin hydrolase substrate channels 3D structure substrate transport starch |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《广西科学》浏览原始摘要信息 |
| 点击此处可从《广西科学》下载免费的PDF全文 |
|
