| 2-(3,4-二甲氧基苯基)-4,5-二苯基-1H-咪唑与DNA相互作用的荧光光谱研究 |
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| 引用本文： | 张立新,叶玲,庞利玲,吴翚. 2-(3,4-二甲氧基苯基)-4,5-二苯基-1H-咪唑与DNA相互作用的荧光光谱研究[J]. 淮阴师范学院学报(自然科学版), 2009, 8(3): 231-234 |
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| 作者姓名： | 张立新 叶玲 庞利玲 吴翚 |
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| 作者单位： | 徐州师范大学,化学化工学院,江苏,徐州,221116;泗阳县环境监测站,江苏,宿迁,223700 |
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| 基金项目： | 国家自然科学基金项目,江苏省自然科学基金项目 |
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| 摘 要： | 运用荧光猝灭法研究了2-(3,4-二甲氧基苯基)-4,5-二苯基-1H-咪唑(I)与鲱鱼精DNA的相互作用,用Stern-Volmer方程和Lineweaver-Burk双倒数函数方程对数据进行了处理.结果表明,两者之间形成了配合物,且得到了319 K时的静态猝灭结合常数(KLB=80.74 L.g-1)和结合位点数(n=0.78254).
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| 关 键 词： | 2-(3,4-二甲氧基苯基)-4 5-二苯基-1H-咪唑 鲱鱼精DNA 荧光猝灭 相互作用 |
A Study on the Interaction Between 2-(3,4-dimethoxyphenyl)-4,5-dipheny-1H-imidazole and DNA by Fluorescence Spectrum |
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| ZHANG Li-xin,YE Ling,PANG Li-ling,WU Hui. A Study on the Interaction Between 2-(3,4-dimethoxyphenyl)-4,5-dipheny-1H-imidazole and DNA by Fluorescence Spectrum[J]. Journal of Huaiyin Teachers College(Natrual Science Edition), 2009, 8(3): 231-234 |
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| Authors: | ZHANG Li-xin YE Ling PANG Li-ling WU Hui |
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| Abstract: | The interaction between 2-(3,4-dimethoxyphenyl)-4,5-dipheny-1H-imidazole(I) and Herring Sperm DNA was studied using fluorescence quenching spectrum.The fluorescence quenching data at 319K were analyzed according to Stern-Volmer equation and Lineweaver-Burk double-reciprocal equation.The results indicated that the complex compound was formed between 2-(3,4-dimethoxyphenyl)-4,5-dipheny1H-imidazole and DNA,the binding constant of static quenching of the reaction (KLB=80.74 L/g) and the binding sites number(n=0.78254) were obtained. |
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| Keywords: | 2-(3,4-dimethoxyphenyl)-4,5-diphenyl-1H-imidazole DNA fluorescence quenching interaction |
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