| 兔生长激素cDNA克隆和表达 |
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| 引用本文： | 董宇清,李荣贵,赵进东,吴光耀. 兔生长激素cDNA克隆和表达[J]. 北京大学学报(自然科学版), 2000, 36(2): 199-202 |
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| 作者姓名： | 董宇清 李荣贵 赵进东 吴光耀 |
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| 作者单位： | 北京大学生命科学学院,北京,100871;北京大学生命科学学院,北京,100871;北京大学生命科学学院,北京,100871;北京大学生命科学学院,北京,100871 |
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| 摘 要： | 从兔脑下垂体中的mRNA中,利用合成引物逆转录PCR,获得了前体和成熟生长激素的cDNA,成熟生长激素的cDNA克隆在pET-3a表达载体并转化到大肠杆菌BL21(DE3 plys)中,获得了高效表达,表达量达到大肠杆菌总蛋白的40%。
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| 关 键 词： | 兔生长激素 cDNA克隆 表达 |
| 收稿时间： | 1999-01-12 |
Cloning and Expression of the Rabbit Growth Hormone cDNA |
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| DONG Yuqing,LI Ronggui,ZHAO Jindong,WU Guangyao. Cloning and Expression of the Rabbit Growth Hormone cDNA[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2000, 36(2): 199-202 |
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| Authors: | DONG Yuqing LI Ronggui ZHAO Jindong WU Guangyao |
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| Affiliation: | College of Life Sciences, Peking University, Beijing, 100871 |
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| Abstract: | The cDNA of preprotein and mature protein of rabbit hypophysiscerebri was obtained. The cDNA of mature growth hormone was cloned in pET|3a, then the recombinant expression vector was transformed into E.coli BL21(DE3 plys).The overexpression was performed and the yield was 40% of the total protein. |
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| Keywords: | rabbit growth hormone cDNA clone expression |
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