530 nm和632.8 nm波长光诱发血液的荧光光谱对比分析

取健康人静脉血,分别用530nm和632．8nm波长光照射血液并检测其荧光光谱。结果显示,这2种波长光在血液中所激发的荧光光谱结构有很大不同。530nm光引发的荧光辐射强度比较大;632．8nm波长光所产生的发射光谱在原激发光的长波和短波2个方向都有分布。这一结果提示,上述2种波长光与血液相互作用的过程有所不同,因而其对血液的生物效应也会有所差异,这对于进一步研究激光血液照射疗法的治疗机理具有一定的意义。

Analysis of Fluorescence Spectra of Blood Induced by Lights of 530 nm and 632.8 nm

The fluorescence spectra from venous blood of normal human are studied upon photoexcition at 530 nm and 632.8 nm respectively.Experimental results show that the spectral profiles of blood induced by these two lights are rather different.Blood can emit much stronger fluorescence when induced by light at 530 nm.And the fluorescence spectra exist both sides of exciting light when photoexcited at 632.8 nm.These results suggest that the interactions between these two wavelengths light and blood are different,thus the biological effects of different wavelengths light to blood are different.This paper may be useful to further understanding the mechanism of Intravascular Low level Laser Irradiation Therapy (ILLLIT).