DNA与CdS-COOH-EcoRI复合物相互作用的研究

采用荧光光谱、原子力显微镜、琼脂糖凝胶电泳等技术探究CdS-COOH-EcoRI复合物与DNA的相互作用.研究发现,小粒径的CdS-COOH-EcoRI复合物与DNA除了有特异性结合,还发生非特异性结合.DNA相对浓度比较大以及恒温孵育时间较短时,以特异性结合为主;而随着DNA相对浓度的减小以及恒温孵育时间的延长,会伴有非特异性结合.Ca2+存在时,可以通过延长孵育时间“激活”酶切反应,使得DNA被剪切.因此可以通过改变DNA相对浓度和孵育时间来调控EcoRI对DNA的剪切.

The Study on the Interaction between DNA and CdS-COOH-EcoRI Complex

In this work,the interaction between CdS-COOH-EcoRI complex and DNA were studied with a combination of fluorescence spectroscopy,atomic force microscopy,agarose gel electrophoresis technology.In the study,it was found that in addition to specific combination there also exists nonspecific combination.Specific combination is dominant in the condition of relatively low DNA concentration and short thermostatic incubation time.However,nonspecific combination also occurs with the decrease of the relative DNA concentration and thermostatic incubation time.If there exists Cacationic in the solution,the incubation time can be prolonged to “activate” enzyme reaction,thus making the DNA sheared.From this,the relative DNA concentration and incubation time were allowed to alter so as control the shearing of DNA by EcoRI.