红豆草细胞悬浮培养中体细胞胚发生的细胞学研究

Somatic Embryogenesis from Cell Suspension Culture of Onobrychis Viciaefolia Scop

The callus originated from the hypocotyl of Cnobrychis viciaefoliaScop.was suspended and shaked on a shaking machine.The isolatedsingle cells obtained from the shaking were then transferred to thinlayerLS liquid menium.During the later perio of suspension culture,it wasobserved that(1)the cyclosis was obvious and it took about 20 minutesfor one cirele;(2)some single cells were active in synthesis DNAduring the initial stage of suspension;(3)a few single cells startedto divide in about 72 hours and amitosis could be seen;(4)somaticembryogenesis could be also seen between 10-30 days;(5)a lot ofembryogenic cell masses were formed after 40 days of culture,and whentransferred to solid-medium they would differentiate into shoots at ahigh freqoency.