人食管癌癌旁粘膜培养上皮生长特性的研究

人食管癌癌旁组织12例和正常食管粘膜15例组织块在盖玻上用199培养基培养,并掺入放射性氘胸腺嘧啶核苷.用光镜、显微分光光度计,放射自显影,扫描电镜检查移植块生长上皮层的生长速度,细胞核 DNA 含量,DNA 合成和形态学改变。结果是前三周,癌旁粘膜组织块培养上皮的生长率,细胞 DNA 含量和 DNA 合成高于食管正常粘膜.第四周以后两组上皮出现生长率降低,分化增加现象,在此培养中未能见到癌旁粘膜上皮转化为恶性细胞.本实验正常粘膜的培养,其生长的上皮细胞可以作为食管癌致癌因素研究的模型.

Biological Characteristic Study on Tissue Culture of Paracancerous Mucosa from Esophageal Carcinoma

Explants of paracancerous mucosa from 12 cases of human esophageal carcinoma (Group Ⅰ) and 15 cases of normal mucosa (Group Ⅱ) were cultured on cover glass in 199 medium and were incorporated with 3H-TdR.The growth rate,DNA content,DNA synthesis and mophological changes of cell outgrowths were examined by LM,microspectrophotometry,autoradiography and SEM. In the first three weeks,the growth rate,the DNA content and the DNA synthesis of cultural cell outgrowth from explants of paracancerous mucosa were higher than those of the normal mucosain the esophagus.After the fourth week, he epithelial cells of both groups showed decrease in the growth rate and showed increase in cellular differentiation.It could not be proved in vitro that the epithelium of precancerous lesions would transform into malignant cells.This study also showed that the cultural epithelial outgrowth from human normal esophageal mucosa could be established usa mode for studying carcinogenesis of esophageal carcinoma in vitro.