| 暗纹东方鲀线粒体ATPase8和ATPase6基因的克隆与序列分析 |
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| 引用本文： | 邵爱华,杜建,陈葵,朱江.暗纹东方鲀线粒体ATPase8和ATPase6基因的克隆与序列分析[J].苏州科技学院学报(自然科学版),2010,27(1):41-46. |
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| 作者姓名： | 邵爱华 杜建 陈葵 朱江 |
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| 作者单位： | 1. 苏州科技学院化学与生物工程学院,江苏,苏州,215009;苏州大学医学部,江苏,苏州,215123
2. 苏州大学医学部,江苏,苏州,215123 |
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| 基金项目： | 农业部行业标准资助项目，苏州科技学院科研基金资助项目 |
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| 摘 要： | 克隆并测定了暗纹东方鲍（Takifugu fasciatus）线粒体ATP合酶Fo亚基8（ATPase8）和亚基6（ATPase6）的序列，并对其进行了初步分析。结果表明：PCR扩增产物的总长度为923bp，其中842bp为ATP8和ATP6基因的编码区。ATP8基因长168bp，编码55个氨基酸残基的蛋白质，其蛋白分子质量为6．8KD，等电点为7．85；ATP6基因长684bp，编码227个氨基酸残基的蛋白质，其蛋白分子质量为24．9KD，等电点为9．16。暗纹东方纯ATP合酶Fo亚基8和亚基6与其他已报道的部分东方纯属鱼类具有很高的同源性。通过探讨ATP合酶F0亚基所含的信息量，发现相对于其他线粒体基因，ATPase8和ATPase6基因所含鉴别信息较少。
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| 关 键 词： | 暗纹东方纯 线粒体DNA ATP合酶 亚基 |
Cloning and sequence analysis of the ATP Synthase Fo Subunits (ATPase8 and ATPase6) genes of mitochondrial DNA genome from Takifugu fasciatus |
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| SHAO Aihua,DU Jian,CHEN Kui,ZHU Jiang.Cloning and sequence analysis of the ATP Synthase Fo Subunits (ATPase8 and ATPase6) genes of mitochondrial DNA genome from Takifugu fasciatus[J].Journal of University of Science and Technology of Suzhou,2010,27(1):41-46. |
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| Authors: | SHAO Aihua DU Jian CHEN Kui ZHU Jiang |
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| Institution: | SHAO Aihua1,2,DU Jian2,CHEN Kui2,ZHU Jiang2(1.School of Chemistry , Bioengineering,SUST,Suzhou 215009,China,2.School of Life Sciences,Soochow University,Suzhou 215123,China) |
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| Abstract: | Mitochondrial ATP synthase Fo subuitit 8(ATPase8) and subunit 6(ATPase6) genes in liver tissue of Takifugu fasciatus were cloned,sequenced and analyzed preliminarily with primers designed according to ATP8 and ATP6 genes of Takifugu rubripes.The results show that the full length of the PCR product is 923 bp which contains 842 bp ORF encoding the ATP8 and ATP6 genes with a 10 bp overlap between them.The ATP8 sequence is 168 bp encoding a protein with 55 amino acid residues whose molecular weight is 6.8 KD an... |
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| Keywords: | Takifugu fasciatus mitochondrial DNA ATP synthase subunit |
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