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| 黑线鳕鱼核酸标准样品的研制 | |
| 引用本文： | 蒋丹,刘宇,丁健,马敏敏,孟菊,黄耀江. 黑线鳕鱼核酸标准样品的研制[J]. 科技导报(北京), 2017, 35(9): 100-106 |
| 作者姓名： | 蒋丹 刘宇 丁健 马敏敏 孟菊 黄耀江 |
| 作者单位： | 1. 辽宁出入境检验检疫局检验检疫技术中心, 大连 116001; 2. 中央民族大学北京市食品环境与健康工程技术研究中心, 北京 100081; 3. 内蒙古通辽市扎鲁特旗动物疫病预防控制中心, 通辽 029100; 4. 中纺粮油(湛江)工业有限公司, 湛江 524012 |
| 基金项目： | 质检公益性行业科研专项（2013IK291）；教育部新世纪优秀人才项目（NCET-11-0842）；统筹推进一流大学和一流学科建设经费（10301－0150200604）；中央民族大学学术团队建设项目（2015MDTD25C&2015MDTD13C） |
| 摘 要： | 为推进鱼类物种鉴定，填补黑线鳕鱼核酸标准样品国内空白，本研究制备了黑线鳕鱼核酸标准样品。研究采用8家单位合作定值的方法，确定了该标准品的DNA质量为（2.02±0.07）μg，k=2；DNA纯度为（1.82±0.08），k=2。并对该标准品的均匀性、稳定性进行了测试，结果表明该样品均匀性与稳定性均达到国家标准样品的技术要求，可用于黑线鳕鱼物种鉴定中方法验证和质量控制。 |
| 关 键 词： | 黑线鳕鱼 物种鉴定 DNA标准样品 均匀性 稳定性 不确定度 |
| 收稿时间： | 2016-10-21 |
The development of a Melanogrammus aeglefinus DNA standard sample |
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| JIANG Dan,LIU Yu,DING Jian,MA Minmin,Meng Ju,HUANG Yaojiang. The development of a Melanogrammus aeglefinus DNA standard sample[J]. Science & Technology Review, 2017, 35(9): 100-106 | |
| Authors: | JIANG Dan LIU Yu DING Jian MA Minmin Meng Ju HUANG Yaojiang |
| Affiliation: | 1. Inspection and Quarantine Technology Center of Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China; 2. Beijing Engineering Research Center of Food Environment and Public Health in Minzu University of China, Beijing 100081, China; 3. Inner Mongolia Animal Disease Prevention and Control Center of Jarud, Tongliao 029100, China; 4. Chinatex Grains & Oils (Zhanjiang) Industries Co.,LTD., Zhanjiang 524012, China |
| Abstract: | To help identify marine fish and provide a reference standard for identification of Melanogrammus aeglefinus, a standard sample for Melanogrammus aeglefinus is established in this study. With collaboration of eight labs, concentration and quality of DNA are tested. The concentration value is (2.02±0.07) μg (k=2) and the purity value of DNA is 1.82±0.08 (k=2) which indicates good quality of samples. PCR and electrophoresis are used to test the uniformity, stability and value analysis of the samples. The results show that these samples in this study meet the technical requirements of national standards and that the method can be used for calibration and quality control of Melanogrammus aeglefinus. |
| Keywords: | Melanogrammus aeglefinus molecular identification standard sample homogeneity stability uncertainty |
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