| 基于EF1-a基因对常见禾本科作物的聚类分析 |
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| 引用本文： | 押辉远,李卫涛,王卫东,焦贞.基于EF1-a基因对常见禾本科作物的聚类分析[J].郑州大学学报(自然科学版),2012(2):108-113. |
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| 作者姓名： | 押辉远 李卫涛 王卫东 焦贞 |
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| 作者单位： | [1]洛阳师范学院生命科学系,河南洛阳471022 [2]郑州大学河南省离子束生物工程重点实验室,河南郑州450052 |
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| 基金项目： | 国家自然科学基金资助项目,编号30800204. |
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| 摘 要： | 基于EF1-a基因核苷酸序列和氨基酸序列,利用分子进化遗传分析软件MEGA 5.0构建常见禾本科植物的最小进化法（ME）、邻接法（NJ）和算术平均数的非加权成组配对法（UPGMA）分子系统进化树.研究结果表明,EF1-a cDNA序列和氨基酸序列的聚类分析都能够反映所列禾本科植物的属间关系,3种进化树的聚类分析分支情况基本一致.基于EF1-a基因的cDNA序列和氨基酸序列构建的ME、NJ树适合用来研究禾本科作物种间或种内的遗传关系,而构建的氨基酸序列UPGMA树有待更多的数据研究.
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| 关 键 词： | 延伸因子EF1-a 禾本科作物 分子系统进化树 |
Cluster Analysis of Some Common Gramineous Plants Based on the Sequences of EFI-a |
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| YA Hui-yuan LI Wei-tao,WANG Wei-dong,JIAO Zhen.Cluster Analysis of Some Common Gramineous Plants Based on the Sequences of EFI-a[J].Journal of Zhengzhou University (Natural Science),2012(2):108-113. |
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| Authors: | YA Hui-yuan LI Wei-tao WANG Wei-dong JIAO Zhen |
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| Institution: | 1. College of Life Sciences, Luoyang Normal University, Luoyang 471022, China ; 2. Henan Key Lab of Ion-beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China) |
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| Abstract: | By means of MEGA 5.0, three kinds of molecular system evolutionary trees （ ME, NJ and UP- GMA） of common gramineous plants were established based on EFI-a genome nucleotide sequences and amino acid sequences. The results showed that the cluster analysis of both EFI-a genome nucleotide se- quences and amino acid sequences could reflect the relationship among gramineous plant genera, and the cluster analysis branches of three kinds of evolutionary trees were almost the same. So it was concluded that ME and NJ trees established on the basis of cDNA sequences and amino acid sequences were appli- cable for the evolutionary relationship among and inside gramineous plant genera, but the UPGMA tree established on the basis of amino acid sequences needed further research. |
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| Keywords: | elongation factor EFI-a gramineous plant molecular system evolutionary tree |
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