高选择性、高灵敏的咔唑衍生物F-荧光增强型探针

以4-羟基咔唑为原料，合成了一种含硅氧基团的咔唑衍生物(HCTC)，通过核磁共振氢谱和高分辨质谱对其结构进行了表征，并对探针的F-检测性能进行了研究。结果表明:HCTC能够利用F-对硅氧键的高亲核性的特异性进攻，表现出对F-的化学反应型荧光探针特性；当加入F-以后，该探针溶液在波长433 nm处出现新的荧光发射峰，并伴随轻微的红移现象，而加入其他常见的阴离子都不能引起类似的现象，HCTC对F-的检测限相对较低(0.21 μmol/L)；将HCTC探针制备成荧光试纸，它能够对含少量乙腈的水溶液中的F-具有荧光检测性能。HCTC是一种高选择性、高灵敏度的F-荧光增强型探针。

The Highly Selective and Sensitive Carbazole Derivative F- Fluorescence-enhanced Probe

A carbazole derivative (HCTC) containing siloxy groups was synthesized using 4-hydroxycarbazole as the raw material. The structure was characterized with 1H NMR and MS, and the F- detection performance of the probe was studied. The results showed that HCTC could take advantage of the nucleophilicity of F- to specifically attack the silicon-oxygen bond, showing the characteristics of a chemically reactive fluorescent probe towards F-. Fluorescence test results showed that when F- was added, a new fluorescence emission peak appeared in the probe solution at the wavelength of 433 nm, accompanied by a slight red shift, while the addition of other common anions could not cause fluorescence enhancement. In addition, the detection limit of F- by HCTC was relatively low (0.21 μmol/L). Finally, the HCTC probe was prepared into a fluorescent test paper, which could detect F- in an aqueous solution containing a small amount of acetonitrile. HCTC can be a fluorescence-enhanced probe with high selectivity and sensitivity.