玉米淀粉双酶水解糖米根霉L - 乳酸发酵的研究

以米根霉（Rhizopus oryzae)NRRL395HS99为菌种，玉米淀粉双酶水解糖为碳源，发酵培养基为基础在摇瓶上确立了最佳发酵条件.并以此为参照进行了50L罐的发酵试验，比较了摇瓶和50L罐发酵的异同。在50L罐中，玉米淀粉双酶水解糖130g/L左右、以重质碳酸钙为中和剂、硅油为消泡剂发酵58－59h可产酸110g/L以上，转化率达87％左右。重质碳酸钙应用于米根霉L－乳酸发酵对于降低生产成本具有重要意义。

THE STUDY OF L-LACTIC ACID FERMENTATION BY RHIZOPUS ORYZAE FROM HYDROLYSATE OF CORN STARCH

Rhizopus oryzae NRRL395HS99 being used as fermentation strain, hydrolysate of corn starch as carbon source, and the fermentation culture medium as basis, the best fermentation conditions were established, and with these as refereuce, the fermentation was studied in a 50 L jar. The differences between fermentation in shake flask and 50 L jar were contrasted. When heavy CaCO 3 was used as neutralization agent and silicon oil as defrother, the 130 g/L of hydrolysate of corn starch,fermented in 50 L jar for 58-59 h, can produce L-lactic acid above 110 g/L and the yield is about 87%. The use of heavy CaCO 3 in the fermentation of L-lactic acid by Rhizopus oryzae has important significance in lowering the cost of production.