| 四种昆虫病毒基因组DNA限制性内切酶图谱分析 |
| |
| 引用本文： | 孟冬丽,张伟,艾秀莲.四种昆虫病毒基因组DNA限制性内切酶图谱分析[J].新疆师范大学学报(自然科学版),2000,19(1):51-56. |
| |
| 作者姓名： | 孟冬丽 张伟 艾秀莲 |
| |
| 作者单位： | 1. 新疆师范大学生物系,乌鲁木齐,830054
2. 新疆农业科学院微生物所,乌鲁木齐,830012 |
| |
| 摘 要： | 应用限制性内切酶图谱分析，结果ＤＮＡ单分子层和Ｓｏｕｔｈｅｒｎ杂交方法，对黄地老虎颗粒体病毒，甘兰菜粉蝶颗粒体病毒，三叶草夜蛾颗粒体病毒和荨麻蛱蝶核型多角体病毒等四种杆状病毒提取基因组ＤＮＡ用９处限制性内切酶酶切，得到不同的酶切图谱使其在基因型上进行区分，通过ＤＮＡ单分子展层发现其核酸链都呈闭环和超螺旋构型，用＾３２Ｐ标记ＡｕＮＰＶ与ＧＶ杂交，没有发现与ＧＶ的同源性。
|
| 关 键 词： | 杆状病毒 限制性内切酶图谱 DNA单分子展层 |
| 修稿时间： | 1999年11月29 |
THE RESTRICTIVE ENDONUCLEASE ELETROPHORESIS OF FOUR VIRUS GENETIC HOMOLOGOUS DNAS IN INSECTS |
| |
| Meng Dongli,Zhang Wei,Ai Xiulian.THE RESTRICTIVE ENDONUCLEASE ELETROPHORESIS OF FOUR VIRUS GENETIC HOMOLOGOUS DNAS IN INSECTS[J].Journal of Xinjiang Normal University(Natural Sciences Edition),2000,19(1):51-56. |
| |
| Authors: | Meng Dongli Zhang Wei Ai Xiulian |
| |
| Abstract: | By restrictive enzyme map analysisin coopertaion with single molecule unfolding of DNA and hybridization method,we extract from four baculovirus species of AsGV,PbGV,StGVand AuNPV genetic homology DNA with nine restrictive enzyme digested,and obtain different electrophoresis map. By single molecule nil folding,we find that all nucleic acid is close - cycled or shows superhelix configuration; when marking AuNPV with 32P,there is no homology with GV. |
| |
| Keywords: | Baculovirus Restriction enzyme map Single molecule unfolding of DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
