| 多巴胺在L-半胱氨酸修饰玻碳电极上的电化学行为及伏安测定 |
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| 引用本文： | 吴影,李念兵.多巴胺在L-半胱氨酸修饰玻碳电极上的电化学行为及伏安测定[J].渝西学院学报(自然科学版),2011(3):46-48. |
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| 作者姓名： | 吴影 李念兵 |
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| 作者单位： | [1]西南大学化学化工学院,重庆北碚400715 [2]重庆文理学院,重庆永川402160 |
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| 摘 要： | 以L-半胱氨酸作为电极修饰剂,采用循环伏安法研究L-Cys/GC电极的制备和DA在该修饰电极的电化学行为及其测定.DA在pH=6.684的磷酸盐缓冲溶液中,在L-Cys/GC电极上产生一对灵敏的氧化还原峰,峰电位分别为Epa=0.180 V和Epc=0.125 V（vs.SCE）.同时用伏安法测定DA的线性范围为1×10-3～1.0×10-6 mol/L,检出限可低达1.0×10-7mol/L（S/N=3）.对1×10-4 mol/L DA平行测定50次,其相对标准偏差约为2.5%.该电极可望进一步发展为微电极,用于生物活体内的神经递质DA的实际检测.
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| 关 键 词： | 多巴胺 L-半胱氨酸 修饰电极 电化学行为 |
Electrochemical behavior and voltammetric determination of dopamineon L-Cysteine modified glassy carbon electrode |
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| Institution: | WU Ying,LI Nian-bin (1.College of Chemistry and Chemical Engineering,Southwest University,Beibei Chongqing 400715,China; 2.Chongqing University of Arts and Sciences,Yongchuan Chongqing 402160,China) |
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| Abstract: | In this paper,the preparation of L-Cysteine modified glassy carbon electrode is successfully accomplished by cyclic voltammetry with the help of L-Cysteine as a dressing agent.The electrochemical behavior and voltammetric determination of dopamine are studied at the L-Cysteine modified electrode.In pH 6.684 phosphate buffer solution,one pair of oxidation and reduction peaks with E_pa=0.180 V and E_pc=0.125 V（vs.SCE） are obtained.At the same time,the linear range for the determination of dopamine is 1×10-3 ~ 1.0×10-6 mol/L with the detection limit of 1.0×10-7mol/L.The relative standard deviation is 2.5%（n=50,1×10-4 mol/L dopamine）.It shows that the modified electrode is expected to the development of microelectrodes for practical determination of neurotransmitter dopamine in vivo. |
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| Keywords: | Dopamine L-Cysteine modified electrode electrochemical behavior |
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