| 抗铜酿酒酵母铜结合蛋白的纯化及性质研究 |
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| 引用本文： | 邢小云,李明春,侯文强,邢来君.抗铜酿酒酵母铜结合蛋白的纯化及性质研究[J].南开大学学报,2000,33(3):63-66. |
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| 作者姓名： | 邢小云 李明春 侯文强 邢来君 |
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| 作者单位： | 南开大学微生物学系!天津，300071，南开大学微生物学系!天津，300071，南开大学微生物学系!天津，300071，南开大学微生物学系!天津，300071 |
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| 摘 要： | 酿酒酵母Ｃｕ＾２＋抗株ＹＮＤ２１经含一定浓度的氯化铜培养基诱导培养后，收集菌体，破碎细胞，离心后ＳｅｐｈａｄｅｘＧ－５０和ＤＥＡＥ－ｃｅｌｌｕｌｏｓｅ柱层析分离可获得三种铜结合蛋白ＤＥ－１，ＤＥ－２，ＤＥ－３。ＤＥ－１脱盐后（Ｇ－１）经鉴定具有金属硫蛋白的特性：表观分子量约为１８ｋＤ；每分子蛋白含２０个巯基，结合１１个铜原子；氨基酸组成中富含半胱氨酸（１８％）、碱性和酸性氨基酸，而酪氨酸和蛋氨酸含
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| 关 键 词： | 酿酒酵母 硫硫蛋白 铜诱导 结合蛋白 纯化 |
| 修稿时间： | 1998-12-03 |
PURIFICATION AND CHARACTERIZATION OF COPPER-BINDING PROTEIN IN A COPPER-RESISTANT STRAIN OF SACCHAROMYCES CEREVISIAE |
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| Xing Xiaoyun,Li Mingchun,Hou Wenqiang,Xing Laijun.PURIFICATION AND CHARACTERIZATION OF COPPER-BINDING PROTEIN IN A COPPER-RESISTANT STRAIN OF SACCHAROMYCES CEREVISIAE[J].Acta Scientiarum Naturalium University Nankaiensis,2000,33(3):63-66. |
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| Authors: | Xing Xiaoyun Li Mingchun Hou Wenqiang Xing Laijun |
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| Abstract: | Three proteins DE 1,DE 2 and DE 3 were produced from the Cu 2 resistant strain YND21 of Saccharomyces cerevisiae induced with CuCl 2 and isolated by means of Sephadex G 50 and DEAE cellulose column chromatography. The purified Cu binding protein G 1 has the characteristics of metallothioneins:(1) low molecular weight (apparent molecular weight 18000D), (2) high Cu and SH content(each molecule contains 20 thiol groups and combines with 11 copper atoms),(3) amino acid composition rich in cysteine(18%), basic and acidic amino acids but almost free from aromatic amino acids (Tyr)and Met, (4) isoelectric point about 5.13. Purified protein G 1 was shown to be homogeneous by analysis of SDS PAGE and IEF, and largely consistent with the metallothioneins reported. |
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| Keywords: | Saccharomyces cerevisiae metal inductivity metallothionein purification |
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