| 高山杜鹃花苞组织培养和优化体系的建立 |
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| 引用本文： | 王蔚琼,肖建忠,李志斌,胡建敏,白霄霞.高山杜鹃花苞组织培养和优化体系的建立[J].河北科技师范学院学报,2012,26(3):17-22. |
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| 作者姓名： | 王蔚琼 肖建忠 李志斌 胡建敏 白霄霞 |
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| 作者单位： | 1. 河北农业大学园林与旅游学院,河北保定,071001
2. 石家庄市农林科学研究院 |
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| 基金项目： | 河北省科技厅科技支撑计划项目 |
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| 摘 要： | 以高山杜鹃"粉冠博士"的花苞为外植体,对其进行初代培养、继代增殖培养、生根培养和组培苗炼苗移栽的研究,建立高山杜鹃组织培养快速繁殖技术体系。结果表明:花苞外植体最佳诱导培养基为Anderson+IAA 1.0 mg/L+KT 12 mg/L;组培苗继代增殖培养基为Anderson+IAA 1.0 mg/L+KT 3.0 mg/L;组培苗生根培养基为1/2 Anderson+IAA 4.0 mg/L+活性炭3.0 mg/L;组培苗炼苗移栽到进口草炭与珍珠岩体积比为3∶1的基质中,成活率达86%。
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| 关 键 词： | 高山杜鹃“粉冠博士” 花苞 组织培养 优化体系 |
Study on the Bud Tissue Culture and Establishment of Optimization System of Rhododendron |
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| WANG Wei-qiong,XIAO Jian-zhong,LI Zhi-bin,HU Jian-min,BAI Xiao-xia.Study on the Bud Tissue Culture and Establishment of Optimization System of Rhododendron[J].Journal of Hebei Normal University of Science & Technology,2012,26(3):17-22. |
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| Authors: | WANG Wei-qiong XIAO Jian-zhong LI Zhi-bin HU Jian-min BAI Xiao-xia |
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| Institution: | College of Landscape Architecture and Tourism, Agricultural University of Hebei, Baoding Hebei,071001 ; 2 Agriculture and Forestry Science Research Institute of Shijiazhuang;China) |
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| Abstract: | Taking the buds of Rhododendron as explants, this paper explored to establish the technique system of tissue culture of Rhododendron, the＂ Dr. Tjebbe＂, in vitro multiplication, including the initial culture, subculture breeding, stronger nurslings breeding, rooting breeding, and transplantation breeding. The results indicated that the best initiation medium was Anderson ＋ IAA 1.0 mg/L ＋ KT 12 mg/L; the multiplication of shoot clump was Anderson ＋ IAA 1.0 mg/L ＋ KT 3.0 mg./L; rooting culture was 1/2 Anderson ＋ IAA 4.0 mg/L ＋ AC 3.0 rag/L; the survival rate was above 86% when the plantlets were transplanted to the substratum consisting of the peat and the pearl rock 3： 1. |
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| Keywords: | the"Dr Tjebbe" Rhododendron bud tissue culture optimization system |
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