| 高粱BTx623幼苗多酚氧化酶Real-time PCR引物扩增效率的检测 |
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| 引用本文： | 蔡玉笑,张志雯,秦素平,陈于和.高粱BTx623幼苗多酚氧化酶Real-time PCR引物扩增效率的检测[J].河北科技师范学院学报,2012,26(3):23-27. |
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| 作者姓名： | 蔡玉笑 张志雯 秦素平 陈于和 |
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| 作者单位： | 河北科技师范学院生命科技学院,河北秦皇岛,066600 |
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| 摘 要： | 介绍了一种检测实时荧光定量PCR技术引物扩增效率的方法,以期为进一步应用实时荧光定量PCR奠定基础。同时通过实时荧光定量PCR技术,研究高粱BTx623幼苗中的多酚氧化酶基因SbPPO的表达情况,结果发现SbPPO2,SbPPO5,SbPPO6的相对表达量较高,这3个SbPPO可能在高粱BTx623幼苗中起到重要功能。
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| 关 键 词： | 扩增效率 实时荧光定量PCR 高粱 幼苗 多酚氧化酶基因 表达 |
Amplification Efficiency of Primers for Real-time PCR of Polyphenol Oxidase Gene in Seedlings of Sorghum Variety BTx623 |
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| CAI Yu-xiao,ZHANC Zhi-wen,QIN Su-ping,CHEN Yu-he.Amplification Efficiency of Primers for Real-time PCR of Polyphenol Oxidase Gene in Seedlings of Sorghum Variety BTx623[J].Journal of Hebei Normal University of Science & Technology,2012,26(3):23-27. |
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| Authors: | CAI Yu-xiao ZHANC Zhi-wen QIN Su-ping CHEN Yu-he |
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| Institution: | ( College of Life Science and Technology, Hebei Normal University of Science ＆ Technology, Qinhuangdao Hebei ,066600, China) |
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| Abstract: | Real-time PCR was affected directly by the amplification efficiency of primers. The aim of this study was to introduce the method of detecting the amplification efficiency of primers for Real-time PCR, which was important for further application. Meanwhile, the expression of polyphenol oxidase was carried out via real-time PCR. The relative expression of SbPPO2, SbPPO5 and SbPPO6 were higher in seedling of sorghum vaiiety BTx623, which might play an important role in the sorghum development. |
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| Keywords: | amplification efficiency real-time PCR sorghum seedling polyphenol oxidase gene expression |
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