| Physiological responses of osteoblasts to cyclic stretching and the change of intracellular calcium concentration |
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| 作者姓名： | TANGLiling WANGYuanliang等 |
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| 作者单位： | Bio-engineeringCoolege,ChongqingUniversity,Chongqing400044,China |
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| 摘 要： | The development of bone tissues is regulated by mechanical stimulation.Cyclic stretching was applied to the osteoblasts that were delivered from rat calvarie,The results showed that stretching at 500με increased the cell proliferation while loading at 1000με and 1500με inhabited cell growth ,Loading also increased the adhesive force between cells and substrate as well as spreading areas of osteobalsts.Furthermore,the fluorescence probe Fluo-3/AM was used to investigate the effect of stretching stimulation on the intracellular calcium concentration of osteoblasts.The intracellular calcium concentration of osteoblasts that were stretched at 500 με for 5 min was 92.9% higher than the control ,After being treated with the panax ontoginseng saponins,the streteched osteoblasts still expressed 28.6% higher intracellular calcium concentration than that of the control ,which proved that both the influx of extracellualr calcium and the release of intracellular calcium store were involved in the increase of intracellular calcium concentration when osteoblasts responded to the cyclic stretching And the influx of extracellular calcium through transmembrance channel played a main role.
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| 关 键 词： | 成骨细胞 定期拉伸 物理性刺激 细胞内钙浓度 化学刺激 生理反应 骨质密度 |
| 收稿时间： | 2002-10-28 |
Physiological responses of osteoblasts to cyclic stretching and the change of intracellular calcium concentration |
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| Liling Tang,Yuanliang Wang,Li Gu,Shaoxi Cai,Aihua Su.Physiological responses of osteoblasts to cyclic stretching and the change of intracellular calcium concentration[J].Chinese Science Bulletin,2003,48(4):346-350. |
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| Authors: | Liling Tang Yuanliang Wang Li Gu Shaoxi Cai Aihua Su |
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| Institution: | TANG Liling, WANG Yuanliang, GU Li, CAI Shaoxi & SU Aihua Bio-engineering College, Chongqing University, Chongqing 400044, China Correspondence should be addressed to Tang Liling |
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| Abstract: | The development of bone tissues is regulated by mechanical stimulation. Cyclic stretching was applied to the osteoblasts that were delivered from rat calvarie. The results showed that stretching at 500 me increased the cell prolifera-tion while loading at 1000 me and 1500 me inhabited cell growth. Loading also increased the adhesive force between cells and substrate as well as spreading areas of osteobalsts. Furthermore, the fluorescence probe Fluo-3/AM was used to investigate the effect of stretching stimulation on the intra-cellular calcium concentration of osteoblasts. The intracellu-lar calcium concentration of osteoblasts that were stretched at 500 me for 5 min was 92.9% higher than the control. After being treated with the panax ontoginseng saponins, the stretched osteoblasts still expressed 28.6% higher intracellu-lar calcium concentration than that of the control, which proved that both the influx of extracellular calcium and the release of intracellular calcium store were involved in the increase of intracellular calcium concentration when os-teoblasts responded to the cyclic stretching. And the influx of extracellular calcium through transmembrance channel played a main role. |
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| Keywords: | stretching osteoblasts proliferation adhesion intra-cellular calcium |
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