鸡肉参中总三萜含量的测定

目的:采用紫外分光光度法对鸡肉参中总三萜成分的含量进行测定。方法:以齐墩果酸为对照品,以5%香草醛-冰醋酸溶液、高氯酸为显色系统,在480 nm波长处测定样品吸光度。结果:齐墩果酸在0.04~0.24 mg/mL范围内吸光度与含量成良好的线性关系,其回归方程为:A=0.001 5 C+0.002 3r,=0.999 9,平均回收率为99.37%,RSD=1.075%(n=6),所测鸡肉参中总三萜含量以齐墩果酸计为1.41%。结论:该方法操作简便、快速,灵敏度高和重现性好,测定结果准确可靠,可用于鸡肉参的质量控制。

Determination of Triterpenoid in Incarvillea Mairei by Ultraviolet Spectrophotometry

Objective: To establish a method for measurement of total triterpene in Jirousheng(Incarvillea mairei) by Ultraviolet spectrophotometry(UV).Methods: Using oleanolic acid as object,and 5% vanillin-glacial acetic acid solution and perchloric acid as color-developing agent,total triterpenes was measured by spectrophotometry at 480 nm wavelength.Results: The oleanolic acid content showed a good linear relationship with absorbance(r=0.999 9) in the range of 0.04-0.24 mg/mL,and the regression equation was A=0.001 5 C+0.002 3.The average recovery was 99.37% and RSD was 1.075%(n=6),Incarvillea mairei contained 1.41% of total triterpene according to the calculation of oleanolic acid content.Conclusion: The method was simple,rapid,high sensitivity,reproducible and reliable for the quality control of Incarvillea mairei.