五指山不同海拔、光照条件下枫香的RAPD分析

为了解五指山不同海拔下枫香居群之间的遗传差异,用50个随机引物,应用随机扩增多态性DNA(RAPD)方法,分别对7个海拔梯度、相同光照条件下的枫香样品和710m海拔高度的6个枫香样品(3个阴性条件样品和3个阳性条件样品)进行了分子生态学研究.结果发现:生长于海拔100～300m的枫香的扩增谱带与生长于高海拔(400～700m)的略有差异,而在相同海拔(710m)的3个阴性条件样品和3个阳性条件样品间,其扩增谱带没有明显差异.这说明长期低海拔的生长环境对枫香的遗传物质有一定的影响,然而在710m海拔的不同光照条件下,虽然枫香生长表现出巨大差异,但在遗传物质方面却没有发现明显差别.

RAPD Analysis on the Liquidambar formosana at Different Altitudes and Under Different Sunlight

To investigate the genetic differences between the Liquidambar formosana populations at different altitudes and in the same light conditions in Wuzhi Mountain of Hainan,the samples of Liquidambar formosana at 7 altitude levels and the 6 samples(among which,there were 3 samples from shade conditions and 3 from sunlight conditions)from the same altitude(700 m) were made a study in molecular biology by RAPD method,with 50 random primes in use.It was found that there were some differences between the amplified bands of Liquidambar formosana which grew in the place of the altitudes from 100 m to 300 m and in the place of the higher altitudes from 400 m to 700 m,while there was no obvious difference among the amplified bands of Liquidambar formosana's samples(3 samples were from shade conditions and 3 from sunlight conditions)which were from the same altitude(710 m).Thus it indicated that the growing conditions had some effects on the genetic substance of Liquidambar formosana trees when they grew in the conditions of a low altitude for a long time,and when they grew in the conditions with or without sunlight at an altitude of 700 m,although a significant difference occured in the growth of Liquidambar formosana, no obvious difference was found in genetic substance.