| 大肠杆菌菌毛抗原K99基因的亚克隆及核苷酸序列测定 |
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| 引用本文： | 邓光存,王玉炯,许崇波,曾瑾.大肠杆菌菌毛抗原K99基因的亚克隆及核苷酸序列测定[J].宁夏大学学报(自然科学版),2002,23(1):76-78. |
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| 作者姓名： | 邓光存 王玉炯 许崇波 曾瑾 |
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| 作者单位： | 宁夏大学,生物工程系,宁夏,银川,750021 |
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| 基金项目： | 国家“86 3”计划基金资助项目 (0 1 0 6 0 5 0 3) |
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| 摘 要： | 利用PCR技术 ,从含有大肠杆菌菌毛抗原K99基因的质粒pX5K中扩增出 0 .4kb的K99菌毛抗原基因 ,然后将其亚克隆到pGEM T easy载体上 ,并转化至受体菌JM10 9中 ,采用碱性裂解法提取质粒DNA后 ,经NcoI和EcoRⅠ双酶切分析和核苷酸序列分析 ,证明插入的K99基因片段具有正确的核苷酸序列
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| 关 键 词： | 大肠杆菌 K99基因 亚克隆 核苷酸序列 |
| 文章编号： | 0253-2328(2002)01-0076-03 |
| 修稿时间： | 2001年9月24日 |
Subcloning and nucleotide sequencing of K99 antigen gene of Escherichia coli |
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| DENG Guang-cun,WANG Yu-jiong,XU Chong-bo,ZENG Jin.Subcloning and nucleotide sequencing of K99 antigen gene of Escherichia coli[J].Journal of Ningxia University(Natural Science Edition),2002,23(1):76-78. |
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| Authors: | DENG Guang-cun WANG Yu-jiong XU Chong-bo ZENG Jin |
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| Abstract: | A 0.4?kb K 99 antigen gene fragment was amplified by PCR from plasmid pX5K containing K 99 antigen gene of Escherichia coli,then it was inserted to pGEM-T-easy vector and transformated to JM109 receptor strain. The recombinant plasmid was extracted and purified, and was studied in detail by restriction endonuclease and nucleotide sequencing. The results showed that the nucleotide sequence of K 99 antigen gene was correspond to that of Escherichia coli. |
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| Keywords: | Escherichia coli K 99 antigen gene subcloning nucleotide sequence |
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