| 酶电极法检测苹果酸-乳酸发酵过程中乳酸变化 |
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| 引用本文： | 孙士青,杨俊慧,张利群,杨艳,史建国,刘长胜.酶电极法检测苹果酸-乳酸发酵过程中乳酸变化[J].山东科学,2009,22(3):7-10. |
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| 作者姓名： | 孙士青 杨俊慧 张利群 杨艳 史建国 刘长胜 |
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| 作者单位： | 1. 山东省科学院生物研究所,山东省生物传感器重点实验室,山东,济南,250014
2. 山东鲁洲食品集团技术中心,山东,沂水,276400 |
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| 摘 要： | 以固定化L-乳酸氧化酶（1．1．1．27）与H2O2电极构成乳酸酶电极生物传感器，研究了乳酸酶电极分析法的各种分析条件及参数，并与药典标准总酸测定法对比测定了葡萄酒苹果酸-乳酸发酵溶液中L-乳酸的含量。结果表明供试溶液中L-乳酸含量约占总酸量的10％～20％。采用酶电极法测定葡萄酒苹果酸-乳酸发酵溶液中的L-乳酸含量具有专一性高、成本低、分析速度快等优点。
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| 关 键 词： | 葡萄酒 苹果酸-乳酸发酵 L-乳酸 酶电极 生物传感器 |
Determination of L-lactic Acid in Malolactic Fermentation Solution with Enzyme Electrode |
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| SUN Shi-qing,YANG Jun-hui,ZHANG Li-qun,YANG Yan,SHI Jian-guo,LIU Chang-sheng.Determination of L-lactic Acid in Malolactic Fermentation Solution with Enzyme Electrode[J].Shandong Science,2009,22(3):7-10. |
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| Authors: | SUN Shi-qing YANG Jun-hui ZHANG Li-qun YANG Yan SHI Jian-guo LIU Chang-sheng |
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| Institution: | 1.Key Laboratory for Biosensors of Shandong Province;Institute of Biology;Shandong Academy of Sciences;Jinan 250014;China;2.Shandong Luzhou Food Group Co.Ltd.;Yishui 276400;China |
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| Abstract: | We constructed a lactic enzyme electrode biosensor with fixed L-lactic oxidase and a hydrogen peroxide electrode.We employed such a biosensor to determine the content of L-lactic acid in grape wine malolactic fermentation solution by comparison with the contents in Chinese Pharmacopoeia total acids determination.The rate of L-lactic acid and total acids was approximately 10% to 20%. |
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| Keywords: | grape wine malolactic fermentation L-lactic acid enzyme electrode biosensor |
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