HLPC梯度洗脱测定抗荨胶囊中升麻素苷和5-O-甲基维斯阿米醇苷的总含量 |
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引用本文: | 史春山,;姜宗文,;姜楠,;肖秋生.HLPC梯度洗脱测定抗荨胶囊中升麻素苷和5-O-甲基维斯阿米醇苷的总含量[J].长春大学学报,2014(10):1380-1383. |
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作者姓名: | 史春山 ;姜宗文 ;姜楠 ;肖秋生 |
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作者单位: | [1]中国人民解放军第二○八医院四六一临床部,长春130021; [2]吉林省军区门诊部,长春130021 |
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基金项目: | 吉林省中医药管理局科技项目(2012-167) |
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摘 要: | 目的:建立抗荨胶囊(防风蒺藜蝉蜕黄芪当归白鲜皮等)中升麻素苷和5-O-甲基维斯阿米醇苷含量的高效液相色谱的方法。方法:以WondaSil C18-WR(4.6 mm×150mm,5μm PH 1-10)为色谱柱;流动相采用甲醇(A)-0.05%磷酸溶液(B)梯度洗脱:0~6min,40%B,6~17min,45%(B),17~30min,75%(B),升麻素苷和5-O-甲基维斯阿米醇苷检测波长为254nm,流速为1.0mL·min-1,柱温为30℃,进样量为10μl。结果:用梯度洗脱得到了较好的分离效果,升麻素苷18.4~92.0μg/mL之间线性关系良好,r=0.9999550(n=6);5-O-甲基维斯阿米醇苷在22.0~110.0μg/mL之间线性关系良好,r=0.9996565(n=6),平均回收率分别为100.28%和100.56%。结论:该研究同时进行升麻素苷、5-O-甲基维斯阿米醇苷的定量分析,方法专属性强,准确度高,可用于抗荨胶囊中升麻素苷和5-O-甲基维斯阿米醇苷的含量测定。
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关 键 词: | 抗荨胶囊 升麻素苷 -O-甲基维斯阿米醇苷 |
Quantitative Determination of Cimicifugoside and 5-O-omethylvisammios-De in Anti-nettle Capsules by HPLC Gradient Elution |
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Institution: | SHI Chunshan , JIANG Zongwen , JIANG Nan, XIAO Qiusheng (1. 461 Clinic Department, 208 Hospital of the People's Liberation Army, Changchun 130021, China; 2. Outpatient Department of Jilin Province Military Area, Changehun 130021, China) |
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Abstract: | Objective: To establish a high-performance liquid chromatography(HPLC) for simultaneous determination of cimicifugoside and 5-O-methylvisammios-De in anti-nettle capsules(radix saposhnikoviae,caltrop,periostracum cicadae,Astragalus membranaceus,angelica sinensis,dictamnus dasycarpus,etc.). Methods: WondaSil C18-WR(4. 6 mm × 150 mm,5 μm PH 1- 10) is used as the column; Mobile phase has been made gradient elution with(A)- 0. 05% phosphoric acid: 0 - 6min,40% B,6 - 17 min,45%(B),17- 30 min,75%(B),the detection wavelength of cimicifugoside and 5-O-methylvisammios-De is 254 nm,the flow speed is 1. 0mL·min- 1,the column temperature is 30℃,and the injection volume was 10 μl. Results: A better separation result is obtained by the gradient elution. There was a good linear relationship for cimicifugoside from 18. 4 to 92. 0 μg /mL r = 0. 9999550(n = 6) and for 5-Omethylvisammioside from 22. 0 to 110. 0 μg /mL,r = 0. 9996565(n = 6),the average recovery rate is 100. 28 and 100. 56% respectively. Conclusion: The quantitative analysis of determination of cimicifugoside and 5-O-methylvisammios-De is conducted. The established method with high accuracy is specific,which can be used to determine the contents of cimicifugoside and 5-O-methylvisammios-De in anti-nettle capsules. |
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Keywords: | anti-nettle capsules cimicifugoside 5-O-methylvisammios-De |
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