| 金针菇液体深层发酵多糖的提取与测定 |
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| 引用本文： | 孙居民,张巨明,李春,徐小琳.金针菇液体深层发酵多糖的提取与测定[J].石河子大学学报,1999,3(1):36-40. |
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| 作者姓名： | 孙居民 张巨明 李春 徐小琳 |
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| 作者单位： | 石河子大学工学院食品科学系!石河子，832003，石河子大学工学院食品科学系!石河子，832003，石河子大学工学院食品科学系!石河子，832003，石河子大学工学院食品科学系!石河子，832003 |
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| 摘 要： | 金针菇液体深层发酵醪中，母液和菌丝体内分别含有胞外多糖、胞内多糖，在母液中加入一定体积９５ ％ 乙醇，得到胞外粗多糖；菌丝体烘干后，粉碎过筛，水浴提取胞内粗多糖。采用蒽酮比色法测定粗多糖中纯多糖的含量。
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| 关 键 词： | 金针菇 液体深层发酵 胞外多糖 胞内多糖 |
Eaxtraction and Measurement of Polysaccharides in the Liquid Submerged Fermentation of Flammulina Velutipes |
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| Sun Jumin,Zhang Juming,Li Chun,Xu Xiaolin.Eaxtraction and Measurement of Polysaccharides in the Liquid Submerged Fermentation of Flammulina Velutipes[J].Journal of Shihezi University(Natural Science),1999,3(1):36-40. |
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| Authors: | Sun Jumin Zhang Juming Li Chun Xu Xiaolin |
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| Abstract: | In the fermentum of submerged fermentation liquid of Flammulina velutipes,there were extracellular polysaccharides in liquor and intercellular polysaccharides in mycdlium.Crude extracellular polysaccharides could be extracted by adding alcohol of 95 percent with a certain volume into liquor.Moreover,baked mycelium was shattered and sieved,then it was water bathed to extract intracellular polysaccharides.Finally,the percentage of pure polysaccharides in the crude polysaccharides could be obtained by using Anthracenone Assay. |
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| Keywords: | Flammulina velutipes liquid submerged fermentation extracellular polysaccharides intracellular polysaccharides |
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