Dissociated cell suspensions ofCarcinus maenas Y-organs as a tool to study ecdysteroid production and its regulation |
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Authors: | J. Y. Toullec C. Dauphin-Villemant |
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Affiliation: | (1) Laboratoire de Biochimie et Physiologie du Développement, CNRS URA 686, Ecole Normale Supérieure, 46 rue d'Ulm, F-75230 Paris cedex 05, France |
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Abstract: | In vitro ecdysteroid production by dissociated Y-organ cells of the shore crabCarcinus maenas was characterized during short-term incubations. Under optimized conditions (M199 adjusted to crab osmolality and with the addition of 10% foetal calf serum), ecdysteroid production by dispersed cells increased linearly during 4-hour incubations, with little intra-assay variation. 25-deoxyecdysone was mainly produced. PurifiedCarcinus molt inhbiting hormone (CamMIH) produced a dose-dependent inhibition of ecdysteroid production by dispersed cells. The cells were about 50 times more sensitive than whole glands to MIH. Other structurally-related peptides were tested. |
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Keywords: | Ecdysteroid biosynthesis neuropeptide regulation Y-organs crustacea |
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