氯化1-辛基-3-甲基咪唑对EMT6细胞的毒性及其DNA损伤作用

研究了氯化1-辛基-3-甲基咪唑(C8mim]Cl])对EMT6细胞的毒性大小及其可能的遗传机制.不同浓度(0.06、0.25、1.00mmol·L-1)的C8mim]Cl]对EMT6细胞染毒12h,WST-1比色法检测了细胞活力,ELISA方法检测了Bcl-2蛋白的表达,Hoechst 33342染色检测了细胞核形态的变化,单细胞凝胶电泳(SCGE)检测了细胞DNA的损伤,流式细胞仪检测了细胞周期和细胞DNA的含量.结果显示,经C8mim]Cl]染毒后,EMT6细胞活力下降,并且呈剂量依赖关系.当C8mim]Cl]浓度高于0.25mmol·L-1时,与对照相比,差异显著.C8mim]Cl]染毒使Bcl-2蛋白表达下调,引起了细胞核形态改变,造成了DNA的断裂损伤和含量下降,诱导了细胞凋亡.从而可以得出结论,DNA损伤参与了C8mim]Cl]诱导的细胞凋亡.

Cytotoxicity and DNA Damage Caused by 1-octyl-3-methylimidazolium Chloride on EMT6 Cells

Cytotoxicity and underlying genetic mechanisms of 1-octyl-3-methylimidazolium chloride(C8mim]Cl〗]on EMT6cells were studied in the present research.EMT6cells were exposed toC8mim]Cl]at the concentrations of 0.06,0.25,1.00mmol·L-1 for 12h,followed by evaluations of WST-1assay,expression level of Bcl-2by ELISA,nuclear morphological alternation by Hoechst 33342staining,DNA damage by single cell gel electrophoresis(SCGE),and cell cycle and DNA contents by flow cytometry respectively.The results demonstrated thatC8mim]Cl]inhibits EMT6cell growth and decreases their viabilities in a dose-dependent manner after 12hof exposure.Compared to the control group,the difference is significant when the concentrations ofC8mim]Cl]are higher than 0.25mmol·L-1.Our results also reveal thatC8mim]Cl]can down-regulate Bcl-2expression,causes nuclear morphological changes and DNA damage,and induces cell apoptosis.These results suggest that DNA damage may be involved in the apoptosis induced byC8mim]Cl]in EMT6cells.