| 20个红花品种资源的ISSR分析 |
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| 引用本文： | 高武军,刘方,高琳琳,李书粉,邓传良,卢龙斗.20个红花品种资源的ISSR分析[J].河南师范大学学报(自然科学版),2014(2):114-118. |
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| 作者姓名： | 高武军 刘方 高琳琳 李书粉 邓传良 卢龙斗 |
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| 作者单位： | 河南师范大学生命科学学院; |
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| 基金项目： | 河南省重点科技攻关项目(102102310021);河南省教育厅自然基金项目(2010A180010,2010B180016) |
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| 摘 要： | 目的:利用ISSR标记技术分析国内20个主要红花品种遗传多样性,为红花种质资源选育提供参考资料.方法:选用ISSR分子标记法对不同地理区域的20个红花品种基因组DNA进行PCR扩增.结果:20个ISSR引物共产生382条扩增带,其中多态性带366条,多态性条带比例为95.42%.10个品种间的遗传相似系数分布在0.615 2~0.790 6之间,说明不同红花品种间遗传多样性较为丰富.聚类分析结果表明,利用ISSR技术可将来自不同地区的20个红花品种分为5个主要类群.其中,杞县刺红花单独为一类群.其余四个类群均未表现出品种遗传多样性与地理分布的相关性.
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| 关 键 词： | 红花 ISSR 遗传多样性 |
Genetic Diversity of 20 Safflower Cultivars Resources by ISSR |
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| Institution: | ,College of Life Science,Henan Normal University |
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| Abstract: | Objective:Inter-simple sequence repeat(ISSR)molecular markers have the important function in analyzing the genetic relationship,genetic diversity,identifying germplasm resources and genetic mapping.Method:Genome DNA of safflower cultivars were amplified using ISSR primers.Result:382bands were amplified with 20ISSR primers,including 366polymorphic bands.The polymorphic percentage is 95.42%.The range of the genetic similarity coefficient was 0.615 2-0.790 6,indicating a rich genetic diversity of Safflower.UPGMA results showed that 20cultivars were divided into five groups according to the genetic distance,of which Qixian Honghua belongs to the first group.Correlation was not been detected between genetic diversity and geographic distance on other four groups. |
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| Keywords: | safflower ISSR genetic diversity |
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