| Fine mapping of a semidwarf gene sd-g in indica rice（Oryza sativa L.） |
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| 作者姓名： | LIANGGuohua CAOXiaoying SUIJiongming ZHAOXiangqiang YANChangjie YIChuandeng GUMinghong |
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| 作者单位： | JiangsuKeyLaboratoryofPlantFunctionGenomics.YangzhouUniver-sity,Yangzhou225009,China |
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| 摘 要： | The semidwarf gene sd-g which has been usedin indiea rice breeding in southern China is a new one, non-allelic to sd-1. To map sd-g, an F2 population derived fromthe cross between Xinguiaishuangai and 02428 was con-structed. The sd-g was roughly mapped between two mi-crosatellite markers RM440 and RM163, with genetic dis-tances of 0.5 and 2.5 cM, respectively. Then nine new poly-morphic microsatellite markers were developed in this region.The sd-g was further mapped between two microsatellitemarkers SSR5-1 and SSR5-51, with genetic distances of 0.1and 0.3 cM, respectively, while cosegregated with SSR418. ABAC contig was found to span the sd-g locus, the region be-ing delimited to 85 kb. This result was very useful for cloningof the sd-g gene.
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| 关 键 词： | 水稻 半矮生基因 简单基因重复制造 分子制图 BAC contig 遗传距离 |
Fine mapping of a semidwarf gene<Emphasis Type="Italic">sd-g</Emphasis> in indica rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.) |
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| LIANGGuohua CAOXiaoying SUIJiongming ZHAOXiangqiang YANChangjie YIChuandeng GUMinghong.Fine mapping of a semidwarf genesd-g in indica rice (Oryza sativa L.)[J].Chinese Science Bulletin,2004,49(9):900-904. |
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| Authors: | Email author" target="_blank">Guohua?LiangEmail author Xiaoying?Cao Jiongming?Sui Xiangqiang?Zhao Changjie?Yan Chuandeng?Yi Minghong?Gu |
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| Institution: | LIANG Guohua, CAO Xiaoying, SUI Jiongming, ZHAO Xiangqiang, YAN Changjie, YI Chuandeng & GU Minghong Jiangsu Key Laboratory of Plant Function Genomics, Yangzhou Univer-sity, Yangzhou 225009, China |
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| Abstract: | The semidwarf gene sd-g which has been used in indica rice breeding in southern China is a new one, non-allelic to sd-1. To map sd-g, an F2 population derived from the cross between Xinguiaishuangai and 02428 was con-structed. The sd-g was roughly mapped between two mi-crosatellite markers RM440 and RM163, with genetic dis-tances of 0.5 and 2.5 cM, respectively. Then nine new poly-morphic microsatellite markers were developed in this region. The sd-g was further mapped between two microsatellite markers SSR5-1 and SSR5-51, with genetic distances of 0.1 and 0.3 cM, respectively, while cosegregated with SSR418. A BAC contig was found to span the sd-g locus, the region be-ing delimited to 85 kb. This result was very useful for cloning of the sd-g gene. |
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| Keywords: | rice semidwarf gene simple sequence repeat (SSR) marker molecular mapping BAC contig |
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