丝线、羊肠线及结扎道数对大鼠CCI疼痛模型的影响

通过比较丝线、羊肠线2种不同材料和不同结扎道数对大鼠CCI疼痛模型痛阈值的影响，优化筛选出更易在实验过程中普及的技术方法．将42只SD雄性大鼠随机分成7组，包括结扎丝线4道、3道与2道3个组；结扎羊肠线4道、3道与2道3个组和1个假手术组，每组6只大鼠．铬制羊肠线和丝线分别结扎大鼠左侧坐骨神经2道、3道、4道；假手术组只暴露左侧坐骨神经，不进行结扎处理．建模成功后测量各组大鼠的机械性缩足反应阈值（MWT），发现丝线3道、4道组和羊肠线2道、3道、4道组与假手术组和自身基础的MWT有极显著差异（P〈0．01）；假手术对照组、丝线2道组与各组自身基础的MWT之间没有差异（P〉0．05），结扎手术前后的MWT之间差异显著，结扎后第7天、14天和21天的MWT之间没有差异．羊肠线3组之间没有显著性差异（P〉0．05）．由此可得出结论：羊肠线2道、3道、4道组，以及丝线3道、4道组均能产生稳定的神经痛，根据MWT的数值大小和是否出现自噬现象可认为，以羊肠线结扎2道组最好，不但MWT的数值小，且操作简便又节省时间，可作为大鼠CCI建模的首选方法．

Effects of Silk and Chromic Gut Thread and Different Ligation Ring Numbers on CCI Model

In our report, the mechanical pain threshold （MWT） of CCI pain models made with different thread materials and number of ligation rings were compared, and the easier methods of CCI （ chronic constriction nerve injury model） were optimized and screened. Forty-two male SD rats were randomly divided into 7 groups, which were three CCI groups which is 4, 3 and 2 ring silk thread ligation, respectively, three CCI groups which is 4, 3 and 2 ring chromic gut thread ligation, respectively, sciatic nerves in rats of 6 CCI groups were ligated with and one group of sham-operation group （n = 6） ; the left chromic gut and silk thread, respectively, the left sciatic nerves in rats of sham-operation group were exposed without ligation. After the model was established, the MWTs were determined. The results indicated that there are significant differences between two CCI groups of 4, 3 ring silk thread ligation, three CCI groups of 4, 3 and 2 ring chromic gut thread ligation, and one group of sham-op-eration group and their base threshold （P 〈 0.01 ） ; there are no significant differences between sham-operation group, base threshold of each group and CCI group of 2 ring silk thread ligation （ P 〉 0.05 ） ; the MWTs before ligation surgery in CCI groups were different from their MWTs after surgery; the MWTs in CCI groups keep stable on 7, 14 and 21 days after surgery; there are no significant differences between the three chromic gut thread groups （ P 〉 0.05 ）. The three CCI groups of 4, 5 and 2 ring chromic gut thread ligation and two CCI groups of 4, 3 ring silk thread ligation produced stable neuralgia. According to MWT value and whether there were self-bites, the CCI group of 2 ring chromic gut thread ligation was the best model, which was the easiest operation and had low MWT value.