抗蛋氨酸结构类似物突变株的筛选

以北京棒杆菌AS1.299经诱变后获得的高产蛋氨酸突变株N3-10作为出发菌株, 依次采用蛋氨酸结构类似物亮氨酸、 原亮氨酸和乙硫氨酸平板对
诱变后的突变株进行筛选, 得到蛋氨酸高产菌株依次为L3,LN7和E31. 结果表明, 亮氨酸对突变株N3-10的抑制质量浓度为8 g/L, 原亮氨酸对突变株L3的抑制质量浓度为3 g/L, 乙硫氨酸对LN7突变株的抑制质量浓度为3 g/L, 经紫外诱变和3种蛋氨酸结构类似物的筛选, 最终获得蛋氨酸产量最高突变株E31, 产量为1.479 g/L, 比出发菌株N3 10蛋氨酸产量高0.379 g/L. 经5次传代, 产量稳定.

Screening of Methionine Analogue Resistant Mutant

The mutant strain N3-10 mutagenized by Corynebacterium pekinense AS1.299 as starting strain was screened with the flat plate which contained leucine,norleucine or ethionine in turn to obtain high yield methionine mutant strains L3,LN7 or E31.Determined inhibiting concentration of leucine to mutant strain N3-10 was 8 g/L,the inhibiting concentration of norleucine to mutant strain L3 was 3 g/L,the inhibiting concentration of ethionine to mutant strain LN7 was 3 g/L.After UV mutagenesis and screening by three ...