鱼藤素对大鼠H9C2心肌细胞的生存活力和凋亡的影响

为探讨不同浓度的鱼藤素(Deguelin)对大鼠H9C2心肌细胞活力及凋亡的影响。通过将不同浓度(0、10、50、100、500、1 000 nmol / L)的鱼藤素作用于H9C2细胞24 h、48 h、72 h,应用CCK-8 法测定鱼藤素对H9C2细胞存活率的影响；划痕实验检测(0、10、50、100、500、1 000 nmol / L)浓度的鱼藤素作用于H9C2细胞24 h、48 h后,对细胞迁移能力的影响。应用Hoechst 33342 / PI 双染试剂盒检测不同浓度鱼藤素(0、10、50 nmol / L)作用于H9C2细24 h后对细胞凋亡的影响。结果表明：10、50、100、500、1 000 nmol / L的鱼藤素作用H9C2细胞24 h、48 h、72 h后,能明显抑制其存活率,10、50、100、500、1 000 nmol / L的鱼藤素作用H9C2细胞24 h、48 h后,能明显抑制其迁移能力,且呈浓度和时间依赖性；荧光倒置显微镜观察发现鱼藤素在低浓度时便可以诱导H9C2细胞凋亡。可见鱼藤素能够呈时间和浓度依赖性来抑制H9C2细胞的生存活力并诱导其凋亡。

Effect of deguelin on the viability and apoptosis of rat H9C2 cardiomyocytes

In order to investigate the effects of different concentrations of deguelin on the viability and apoptosis of rat H9C2 cardiomyocytes. H9C2 cells were incubated with different concentrations (0, 10, 50, 100, 500, 1 000 nmol.L-1) of deguelin for 24 h, 48 h, 72 h. The CCK-8 method was used to investigate the viability of H9C2 cells with different concentrations (0, 10, 50, 100, 500, 1 000 nmol.L-1) of deguelin; Scratch test was used to investigate the cell migration ability after incubated with different concentrations (0, 10, 50, 100, 500, 1 000 nmol.L-1) of deguelin for 24 h, 48 h; Hoechst 33342/PI double staining kit was used to investigate the effect of different concentrations of deguelin (0, 10, 50 nmol.L-1) on H9C2 cells for 24 h. The results show that after treatment with 0, 10, 50, 100, 500 and 1 000 nmol.L-1 of deguelin for 24 h, 48 h, 72 h the survival rate of H9C2 cells were significantly inhibited; the migration ability of H9C2 cells was significantly inhibited after treatment with concentrations of deguelin (0, 10, 50, 100, 500 and 1 000 nmol.L-1) for 24 h, 48 h. Those changes were in a concentration- and time-dependent manner. It is concluded that deguelin can inhibit the viability and induce apoptosis of H9C2 cells in a time- and concentration- dependence.