| 人类疱疹病毒7 U41蛋白DNA链结合能力分析 |
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| 引用本文： | 张立伐,唐永煌,邹平,山西弘一. 人类疱疹病毒7 U41蛋白DNA链结合能力分析[J]. 暨南大学学报(自然科学与医学版), 2002, 23(2): 56-58 |
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| 作者姓名： | 张立伐 唐永煌 邹平 山西弘一 |
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| 作者单位： | 暨南大学医学院第一附属医院感染科,广东,广州,510630;大阪大学医学部微生物科,日本,大阪,565-0871 |
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| 摘 要： | 目的 :对人类疱疹病毒 7(humanherpesvirus 7,HHV - 7)U4 1的核酸结合能力进行分析鉴定。方法 :DNA结合蛋白分离收集、免疫沉淀及Western印迹检测U4 1蛋白对单、双链DNA的不同结合能力。结果 :U4 1蛋白能结合单链DNA而不能结合双链DNA。结论 :U4 1的功能之一是保持DNA模板的合适构型 ,以便DNA的延伸和病毒DNA的合成。
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| 关 键 词： | 人类疱疹病毒 开放读框 U41功能 |
| 文章编号： | 1000-9965(2002)02-0056-03 |
Identification of the DNA binding ability of human her pesvirus 7 U41 protein |
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| ZHANG Li-fa ,TANG Yong-huang ,ZOU Ping ,YAMANISHI Koichi. Identification of the DNA binding ability of human her pesvirus 7 U41 protein[J]. Journal of Jinan University(Natural Science & Medicine Edition), 2002, 23(2): 56-58 |
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| Authors: | ZHANG Li-fa TANG Yong-huang ZOU Ping YAMANISHI Koichi |
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| Affiliation: | ZHANG Li-fa 1,TANG Yong-huang 1,ZOU Ping 2,YAMANISHI Koichi 2 |
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| Abstract: | Aim: To identify the DNA binding ability of human herpesvirus 7(HHV-7) U41 protein. Methods: DNA binding assay and Western blot were done to detect the single and double DNA strands binding ability of U41 protein. Results: the U41 protein was able to bind to a single-strand DNA cellulose column but not to double-strand DNA cellulose column. Conclusion: These findings strongly suggest that the major function of U41 is to keep the DNA template in the optimal conformation for DNA elongation and be required for viral DNA synthesis. |
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| Keywords: | human herpesvirus open reading frame U41 function |
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