| 一种新的高灵敏测定抗坏血酸的荧光分析法 |
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| 引用本文： | 李晓燕,张元勤,王磊.一种新的高灵敏测定抗坏血酸的荧光分析法[J].四川大学学报(自然科学版),2003,40(3):546-551. |
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| 作者姓名： | 李晓燕 张元勤 王磊 |
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| 作者单位： | 乐山师范学院环境与生命科学系,四川,乐山,614000 |
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| 基金项目： | theEducationalCommitteeofSichuanProvinceofChina(2 0 0 0 B2 9) |
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| 摘 要： | 提出了一种新的高灵敏测定抗坏血酸的荧光分析法.抗坏血酸与Cu2+在pH为7.40的磷酸盐缓冲溶液中生成羟基自由基,然后羟基自由基与苯甲酸反应形成荧光物质.建立了测定抗坏血酸的适合条件,测定抗坏血酸的线性范围为(0.0128～1.92)
×10-6g/mL,方法的检测限是7.75×10-9g/mL.对于8.56 ×10-7g/mL的抗坏血酸测定6次,其相对标准偏差为1.6%.
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| 关 键 词： | 抗坏血酸 荧光分析 羟基自由基 羟基化苯甲酸 |
A new Highly Sensitive Spectrofluorimetric Method for the Determination of Ascorbic Acid |
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| Abstract.A new Highly Sensitive Spectrofluorimetric Method for the Determination of Ascorbic Acid[J].Journal of Sichuan University (Natural Science Edition),2003,40(3):546-551. |
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| Authors: | Abstract |
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| Abstract: | A new spectrofluorimetric method for determining ascorbic acid was proposed by the athors. First, the athors let ascorbic acid react with Cu2+ ions to form a hydroxyl radical in phosphate buffer at pH7.4 , and then let the hydroxyl radical react with benzoic acid to form a fluorescent product. The optimal experimental determining conditions were established. The linear range of this method was (0.0128～1.92) ×10-6g/mL of AA. The detection limit was calculated to be 0.00775×10-6g/mL . The relative standard deviation of this method was 1.6 % at 0.856 ×10-6g/mL for 6 runs. |
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| Keywords: | ascorbic acid spectrofluorimetric hydroxyl radical hydroxylate benzoate |
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