| 裂叶秋海棠的离体快速繁殖 |
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| 引用本文： | 唐荣华,李云飞,王丽.裂叶秋海棠的离体快速繁殖[J].四川大学学报(自然科学版),2004,41(3):637-640. |
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| 作者姓名： | 唐荣华 李云飞 王丽 |
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| 作者单位： | 四川大学生命科学学院,成都,610064 |
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| 摘 要： | 利用野生裂叶秋海棠叶片及叶柄培养，通过两种方式获得大量再生植株。(1)直接从外植体表面诱导出不定芽，最佳诱导培养基为MS 6-BA 3mg／L，不定芽在培养基MS NAA0．5mg／L 6-BA0．5mg／L增殖成丛生芽．(2)先诱导出愈伤组织，再由愈伤组织分化出大量不定芽．最佳愈伤组织诱导培养基为MS NAA0．5mg／L 2，4-D0．5mg／L 6-BA0．5mg／L,愈伤组织不定芽分化培养基为MS 6-BA 1mg／L,试管苗在含1／5MS无机盐的培养基上生根，之后移栽成活率达85％。
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| 关 键 词： | 裂叶秋海棠 不定芽 愈伤组织 快速繁殖 |
| 文章编号： | 0490-6756(2004)03-0637-04 |
| 修稿时间： | 2002年10月17 |
The Rapid Propagation in Vitro of Begonia laciniata |
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| TANG Rong-hua,LI Yun-fei,WANG Li.The Rapid Propagation in Vitro of Begonia laciniata[J].Journal of Sichuan University (Natural Science Edition),2004,41(3):637-640. |
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| Authors: | TANG Rong-hua LI Yun-fei WANG Li |
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| Abstract: | A number of plantlets of Begonia laciniata were propagated in two way using leaves and stipes of wild individual.(1) Adventitious buds were directly induced from the explant on MS+6-BA 3mg/L medium.The Adventitious buds could rapidly grow into turfed plantlets on MS+NAA 0.5mg/L+6-BA 0.5mg/L medium.(2) Callus was best induced from lesves of Begonia Laciniata on MS+NAA 0.5mg/L+2,4-D 0.5mg/L+6-BA 0.5mg/L medium. Adventitious buds were easily indued from callus on MS+6-BA 1mg/L medium.The roots of test-tube quikly grew up on medium with 1/5MS inorganic composition,then the test-tube plantlet could be transplanted and lived at a rate of 85% . |
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| Keywords: | Begonia laciniata adventitious bud callus rapid propagation |
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