| 调控深层发酵条件提高果胶酶产率的研究 |
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| 引用本文： | 邵建宁,余秋生,刘海森.调控深层发酵条件提高果胶酶产率的研究[J].甘肃科学学报,2001,13(2):32-34. |
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| 作者姓名： | 邵建宁 余秋生 刘海森 |
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| 作者单位： | 甘肃省科学院生物研究所, |
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| 基金项目： | 甘肃省科技攻关项目!( GZ91 4 -2 -1 9) |
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| 摘 要： | 对利用高产果胶酶突变株G5512和500L发酵罐中调控深层发酵条件提高酶产率进行了试验研究，结果表明，通过分批补料对酶产率有提高，以高酯果胶为底物的酶产率提高7.0%，以低酯果胶为底物酶产率提高23.5%，调节发酵液pH在3.5～4.0之间，以高酯果胶为底物酶产率提高9.7%，以低酯果胶为底物酶产率提高40.9%，添加0.1%吐温80对酶产率没有影响。
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| 关 键 词： | 果胶酶 深层发酵 酶活性 酶产率 固体发酵法 分批补料 PH值 |
| 文章编号： | 1004-0366(2001)02-0032-03 |
| 修稿时间： | 2000年6月20日 |
STUDIES ON THE ENHANCEMENT OF PECTINASE PRODUCTIVITY BY ADJUSTING CONDITIONS OF DEEP FERMENTATION |
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| SHAO Jian-ning,SHE Qiu-sheng,LIU Hai-sen.STUDIES ON THE ENHANCEMENT OF PECTINASE PRODUCTIVITY BY ADJUSTING CONDITIONS OF DEEP FERMENTATION[J].Journal of Gansu Sciences,2001,13(2):32-34. |
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| Authors: | SHAO Jian-ning SHE Qiu-sheng LIU Hai-sen |
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| Abstract: | An experimental study was made to enhance the pectinase productivity by adjusting the conditions of deep fermentation for the high- yield mutantstrain G5 5 1 2 in a5 0 0 liter tank.The results showed that to feed batch culture can enhance enzyme productivity by 7.0 % with high methoxyl pectin as substrate,and by 2 3.5 % with low methoxyl pectin as substrate.When the p H value of fermentation liquor is kept between3.5 and4.0 ,enzyme productivity can be enhanced by9.7% with high methoxyl pectin as substrate,and by40 . 9% with low methoxyl pectin as substrate. Adding Tween 80 ( 0 .1 % ) into the fermentation liquor has no effect on enzyme productivity. |
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| Keywords: | pectinase deep fermentation enzyme activit |
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