| 龙胆毛状根的诱导培养 |
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| 引用本文： | 江洪如,涂艺声.龙胆毛状根的诱导培养[J].江西科学,1995,13(4):244-248. |
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| 作者姓名： | 江洪如 涂艺声 |
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| 作者单位： | 江西省科学院生物资源研究所 |
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| 摘 要： | 发根农杆菌（Ａ．ｒｈｉｚｏｇｅｎｅｓ）Ｒ１５００感染龙胆茎段、叶片外植体，１５ｄ后可诱导出毛状根。茎段诱导率高于叶片，可达４２．３％。毛状根的诱导与温度和光照有关。转化根具有抗卡那霉素的特征，在不含激素的ＭＳ培养基上培养，筛选出了生长正常的毛状根系。
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| 关 键 词： | 龙胆 发根农杆菌 毛状根 诱导培养 药用植物 |
HAIRY ROOTS INDUCED AND CULTURED FROM Gentiana manshurica Kitag |
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| Jiang Hongru, Tu Yisheng, Wang Biqin, Ding Jiannan.HAIRY ROOTS INDUCED AND CULTURED FROM Gentiana manshurica Kitag[J].Jiangxi Science,1995,13(4):244-248. |
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| Authors: | Jiang Hongru Tu Yisheng Wang Biqin Ding Jiannan |
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| Abstract: | Stem and leaf explants of Gentiana manshurica Kitag were infected with Agrobacteium rhizogenes strain R1500 and hairy roots were induced from the infected explants 15 days later.Frequency of stem-induction amounted to 42 . 3 % 'which was higher than that of leaf-induction. Hairy root induction was related to temperature and illumination.Hairy roots grew well in hormore-free MS agar medium and the transformed roots were resistant to kanamycin. |
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| Keywords: | Gentiana maushurica Kitag Agrobacterium rhizogenes hairy root induction |
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