白腐菌的筛选及对松木片木素和树脂的脱除

为评价从野外筛选得到的白腐菌对造纸原料马尾松的木素降解和树脂脱除能力,将带菌样本经室内平板初筛及平板变色反应复筛,得到了20株纯白腐菌菌株,并对变色系数最小的一株菌株G进行松木片固体接种试验,测定3、5、7、9天后松木片的木素、树脂降解情况.结果表明,随着培养时间的延长,松木片失重率逐渐增大,K lason木素和总木素含量逐渐降低,二氯甲烷抽提物脱除率逐渐增大.表面扫描电镜(SEM)照片表明,接种白腐菌的木片细胞壁上产生了明显的腐朽沟槽现象;切片断面显微结构显示,在细胞壁及胞间层有菌丝出现.

Screening of White-Rot Fungi and Degradation of Lignin and Pitch in Masson Pine by White-Rot Fungi

In order to evaluate the capability of lignin degradation and pitch elimination from Masson pine chips by nature white-rot fungi,20 white-rot fungus strains were obtained by petri dishes screening and petri dishes color-reaction screening.One G strain with the lowest Mycel/Col value was then inoculated onto the surface of Masson pine chip,and the degradation rates of lignin and pitch were measured after 3,5,7,9 days,respectively.The results indicate that,with the increase in inoculation time,the losing rate of wood mass and the elimination of methylene chloride extractive gradually increase,while the contents of Klason lignin and the total lignin decrease.It is also indicated by SEM that there exist obvious moldy grooves on the cell walls of Masson pine chip.Moreover,the results of the cross-sectional slice-up study show that hypha develops on the cell walls and the intercellular layers.