牛胚胎体外生产技术的应用研究

利用屠宰牛卵巢抽出的卵母细胞，以卵裂率和囊胚发育为标准，对牛胚胎体外生产过程进行了简化试验，结果显示：不加卵丘细胞的高密度卵母细胞体外成熟（１００－２００枚／平皿）可代替加卵丘细胞标准密度培养，其卵裂率和囊胚发育率没有显著变化，体外授精时间可从成熟培养后２２小时延长至２７小时，卵裂率和囊胚发育率均没有显著变化。卵母细胞体外成熟后可以不经洗涤直接移入受精滴，原成熟培养皿内残留孵丘细胞再培养４８小时形

PRACTICAL STUDY ON IN VITRO PRODUCTION OF BOVINE EMBRYOS

Aseries of experiments was performed to made the technique of in vitro production(IVP)ofbovine embryos simple and effective by estimating the rates of cleavage and blastocyst developedafter IVM and IVF of abatoir-derived oocvtes. The results showed that:(1)Thsin vitro maturation density coud be raised from 50/dish to 100-200/dish without significant changes in the rates of cleavage(62.5%vs 57.6%)and blastocyst developed(29.1%vs 23.7%)(P0.00);(2)The time for in vitro maturation could be extended form 22h to 27h:(3)Washing process before insemination of IVM cocytes was not necessary;(4) The cumulus cells monolayer derived form denuding the in vitro matued oocytes could be substituted for the standard granular cell monolayer to support the development of IVP embryos;(5)The cumulus cell monolayer could be used for the co-culture of IVP embryos more than once. Seven pregnancies were established after the transfer of IVP embryos produced by the simplifiedmethod and 12 heifers and 8 healthy calves were born successfully It is concluded that thetraditional IVP technique of bovine embryos should be simplified in many ways to increaseproduction efficiency.