护肾Ⅰ号对顺铂致肾损伤预防作用的实验研究

目的研究护肾Ⅰ号对顺铂所致贵州小型猪急性肾损伤的预防作用。方法将贵州小型猪随机分为正常对照组、顺铂模型组、中药预防组。每天给中药预防组小型猪灌服护肾Ⅰ号煎剂,连续灌胃5 d,以4 mg/kg顺铂一次性腹腔注射,膀胱插管收集尿液,动态观察给顺铂前后第0、1、3、6、24、489、6 h时段小型猪的尿量,测尿中肌酐及尿素氮浓度,计算各时段肌酐和尿素氮的排出量。结果与正常对照组比较,顺铂模型组尿量和肌酐、尿素氮的排出量显著减少,中药预防组的各项指标得到明显改善。结论护肾Ⅰ号可预防顺铂所致的肾损伤。

Preventive Effects of "Hu-Shen No. 1"on Cisplatin-Induced Nephrotoxicity in Guizhou Miniature Pigs

Objective To study the preventive effects of "Hu-Shen No.1" on acute nephrotoxicity of cisplatin in Guizhou miniature pigs. Methods Guizhou miniature pigs were randomly divided into three groups,namely the control group,the model group and the prevention group.The prevention group received "Hu-Shen No.1" by gavage for five days.After one hour of the 5th administration of "Hu-Shen No.1",animals were given cisplatin(4 mg/kg) by a single peritoneal injection.Urine samples were collected by a bladder fistula.Urinary output,urine level of creatinine and urea nitrogen were dynamically observed on the 1st hour before injection and the 1st,3rd,6th,24th,48th,96th hours after cisplatin treatment.The excretion of creatinine and urea nitrogen were calculated on the basis of the above determination.Results Urinary output,excretion of creatinine and urea nitrogen of the model group were significantly lower than that of the control group.The results of the prevention group were markedly improved. Conclusion "Hu-Shen No.1" may protect kidney against cisplatin-induced nephrotoxicity.