Calcium pretreatment increases thermotolerance of Laminaria japonica sporophytes
—Using Yaluhe farm as an example |
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基金项目: | This work was supported by Opening Foundation of Key Laboratory of Coastal Sea Ecology and Environment, National Maritime Bureau, the National Natural Science Foundation of China (Grant No. 30270258) and Program for New Century Excellent Talents in University (Grant No. NCET 05-0597). |
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摘 要: | Calcium is a secondary messenger in plant signaling, and its concentration changes spatially and temporally during the course of heat stress. In the present study, potassium antimonate was used to visualize calcium localization in blades of a marine macroalga, the juvenile Laminaria japonica sporophytes under heat stress (25℃). Result showed that loosely bound calcium was mainly distributed on the cell wall under normal conditions (10℃), and flowed into the cytoplasm when exposed to heat. The simutaneous assay on the antioxidant system changes was performed. Oxidative damage, as measured by generation of reactive oxygen species (ROS) malondialdehyde (MDA) content, increased significantly during heat stress, and calcium pretreatment alleviated oxidative damage. The assay on the activities of six antioxidant enzymes demonstrated that their enzymatic activities were inhibited when exposed to heat stress, but Ca2+ pretreatment effectively attenuated the inhibition. Results in the present study inferred that calcium homeostasis plays an essential role in L. japonica sporophyte when exposed to heat, and calcium pretreatment could improve its thermo-tolerance.
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关 键 词: | 钙稳态 孢子体 预处理 耐热性 海带 氧化损伤 抗氧化系统 酶活性抑制 |
收稿时间: | 18 April 2008 |
修稿时间: | 18 July 2008 |
Calcium pretreatment increases thermotolerance of Laminaria japonica sporophytes |
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Authors: | You Wang Qingyun Yu Xuexi Tang Lili Wang |
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Institution: | College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, China |
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Abstract: | Calcium is a secondary messenger in plant signaling, and its concentration changes spatially and temporally during the course of heat stress. In the present study, potassium antimonate was used to visualize calcium localization in blades of a marine macroalga, the juvenile Laminaria japonica sporophytes under heat stress (25 °C). Result showed that loosely bound calcium was mainly distributed on the cell wall under normal conditions (10 °C), and flowed into the cytoplasm when exposed to heat. The simutaneous assay on the antioxidant system changes was performed. Oxidative damage, as measured by generation of reactive oxygen species (ROS) malondialdehyde (MDA) content, increased significantly during heat stress, and calcium pretreatment alleviated oxidative damage. The assay on the activities of six antioxidant enzymes demonstrated that their enzymatic activities were inhibited when exposed to heat stress, but Ca2+ pretreatment effectively attenuated the inhibition. Results in the present study inferred that calcium homeostasis plays an essential role in L. japonica sporophyte when exposed to heat, and calcium pretreatment could improve its thermo-tolerance. |
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Keywords: | Calcium localization Calcium pretreatment Heat stress Antioxidant enzyme Laminaria japonica sporophyte |
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