| 菠菜基因组DNA提取方法与条件的研究 |
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| 引用本文： | 丛娟,李晓玲,宫莉,李连贵. 菠菜基因组DNA提取方法与条件的研究[J]. 吉林工学院学报, 2007, 28(4): 467-472 |
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| 作者姓名： | 丛娟 李晓玲 宫莉 李连贵 |
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| 作者单位： | 长春工业大学生物工程学院 吉林长春130012 |
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| 摘 要： | 分别采用CTAB、SDS、高盐低pH三种方法从菠菜叶片中提取基因组DNA,通过A260/A280值、A260/A230值和琼脂糖凝胶电泳对所提取的DNA进行定量、定性分析和综合比较。研究提取缓冲液用量、水浴时间、蛋白质沉淀剂(氯仿-异戊醇)用量、DNA沉淀剂(异丙醇)用量等条件对DNA提取效率和纯度的影响。并对CTAB法的提取条件进行了正交试验,优选出菠菜基因组DNA提取的适宜条件。
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| 关 键 词： | 菠菜 基因组DNA提取 DNA检测 正交试验 |
| 文章编号： | 1006-2939（2007）04-0467-06 |
| 收稿时间： | 2007-03-15 |
| 修稿时间： | 2007-03-15 |
Study on the method and conditions for spinach genomic DNA extraction |
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| CONG Juan,LI Xiao-ling,GONG Li,LI Lian-gui. Study on the method and conditions for spinach genomic DNA extraction[J]. Journal of Jilin Institute of Technology, 2007, 28(4): 467-472 |
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| Authors: | CONG Juan LI Xiao-ling GONG Li LI Lian-gui |
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| Abstract: | Genomic DNA is extracted from the leaves of spinach by means of CTAB,SDS,high salt and low pH.The extracted DNA is tested and analyzed with A260/A280,A260/A230 and agarose electrophoresis.The influences of buffer liquid dosage,water bath time,protein precipitant(chloroform-isopentanol) quantity and DNA precipitant(isopropanol) dosage on the extraction efficiency and purity of DNA are studied.We make the orthogonal test get the optimum condition for DNA extraction. |
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| Keywords: | spinach genomic DNA extraction DNA test orthogonal test. |
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