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| 天门冬氨酸对精氨酸激酶的作用研究 | |
| 引用本文： | 汤洪敏,杨吟野. 天门冬氨酸对精氨酸激酶的作用研究[J]. 贵州大学学报(自然科学版), 2005, 22(4): 388-391 |
| 作者姓名： | 汤洪敏 杨吟野 |
| 作者单位： | 贵州民族学院化学系,贵州,贵阳,550025;云南大学,生命科学学院,云南,昆明,650091;贵州民族学院物理系,贵州,贵阳,550025 |
| 基金项目： | 贵州省科学技术基金资助项目（2004148）. |
| 摘 要： | 应用酶活测定、荧光发射光谱等方法分析了精氨酸激酶在不同浓度天门冬氨酸中活性变化和去折叠动力学。实验结果表明：天门冬氨酸引起精氨酸激酶失活与去折叠，并且过程中没有蛋白质的聚沉；精氨酸激酶的去折叠遵循一级反应动力学的二相过程，包括快相和慢相；失活与天门冬氨酸浓度呈正相关；当去除天门冬氨酸的影响，精氨酸激酶的活力能得到恢复。通过对比实验结果暗示，天门冬氨酸是通过其解离状态的H＋改变了机体pH值而影响精氨酸激酶活性的，并且就天门冬氨酸对精氨酸激酶影响及其意义进行探讨。 |
| 关 键 词： | 精氨酸激酶 天门冬氨酸 pH值 失活 去折叠动力学 |
| 文章编号： | 1000-5269（2005）04-0388-04 |
| 收稿时间： | 2005-07-11 |
| 修稿时间： | 2005-07-11 |
Effect of aspartate on arginine kinase |
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| TANG Hongmin,YANG Yinye. Effect of aspartate on arginine kinase[J]. Journal of Guizhou University(Natural Science), 2005, 22(4): 388-391 | |
| Authors: | TANG Hongmin YANG Yinye |
| Affiliation: | 1. Department of Chemistry, Guizhou University for Ethnic Minorities, Guiyang 550025, China; 2. Department of Physics, Guizhou University for Ethnic Minorities, Guiyang 550025, China; 3. College of Life Science, Yunnan University, Kunming 650091, China |
| Abstract: | The inactivation and unfolding of arginine kinase by aspartic acid was studied by enzyme activity and fluorescence emission spectra. The results showed that aspartic acid caused inactivation and unfolding of arginine kinase with no aggregation, the arginine kinase unfolding corves showed a first-order kinetics with two phases in the aspartic acid solutions, a fast-phase and a slow one. Inactivation of arginine kinase caused by aspartic acid was aspartic acid concentration-dependent. Aspartic acid denatured arginine kinase was reactivated by dilution. The significance and mechanism of arginine kinase active change by aspartic acid have been expounded in this paper. |
| Keywords: | arginine kinase aspartic acid pH inactivation kinetics of unfolding |
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