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| 纤维素分解混合菌群的筛选及其腐解稻草的研究Ⅰ纤维素分解混合菌群的筛选 | |
| 引用本文： | 谢桂先,曾常清,谭周进,刘强,荣湘民.纤维素分解混合菌群的筛选及其腐解稻草的研究Ⅰ纤维素分解混合菌群的筛选[J].世界科技研究与发展,2009,31(2):329-331. |
| 作者姓名： | 谢桂先 曾常清 谭周进 刘强 荣湘民 |
| 作者单位： | 1. 湖南农业大学资源环境学院,长沙,410128 2. 湖南省烈士公园管理处,长沙,410005 3. 湖南中医药大学基础医学院,长沙,410208 |
| 基金项目： | 湖南省自然科学基金，湖南省科学技术厅重点项目 |
| 摘 要： | 为了得到强分解纤维素的复合微生物来配合秸秆还田，从不同生态环境的土壤中用选择性培养基进行了纤维素分解混合菌群的富集，得到了纤维素酶活性较高的混合菌群，该菌群以兼性厌气性细菌为主，能够在较短的时间内溶解滤纸。该菌群利用CMC-Na的能力比滤纸好。以稻草粉为底物时产酶的最佳尿素用量为0．1％，生长曲线表明该菌群在96h开始进入稳定生长期，筛选到了一个能够较好地腐解稻草的混合菌群。 |
| 关 键 词： | 纤维素分解菌 混合发酵 纤维素酶 稻草 |
Study on Screening and Rice-straw Decomposing Function of Cellulose-decomposing Microbes Mixture |
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| XIE Guixian,ZENG Changqing,TAN Zhoujin,LIE Qiang,RONG Xiangmin.Study on Screening and Rice-straw Decomposing Function of Cellulose-decomposing Microbes Mixture[J].World Sci-tech R & D,2009,31(2):329-331. | |
| Authors: | XIE Guixian ZENG Changqing TAN Zhoujin LIE Qiang RONG Xiangmin |
| Institution: | XIE Guixian, ZENG Changqing, TAN Zhoujin, LIU Qiang,RONG Xiangmin (1. College of Resource and Environment, Hunan Agricultural University, Changsha 410128; 2. Management Department of Hunan Hero Park, Changsha 410005 ; 3. School of Preclinical Medicine ,TCM University of Hunan, Changsha 410208 ) |
| Abstract: | In order to obtain excellent cellulose-decomposing complex microorganisms for decomposing rice-straw returned to soil, the studies on the screening of cellulose-decomposing microbes mixture from different ecological soils were conducted. The results showed that an excellent cellulose-decomposing microbial mixture was acquired. It could decompose filter paper in short time and was mainly composed of part-anaerobic bacteria. Its CMC-Na eellulase activities were higher than filter paper cellulase activities. When it was used to decompose rice-straw powder added with 0. 1% urea concentration, the eellulase activities were the highest. The growth curve showed that it began to enter stationary growth period at 96 hours. The cellulose-decomposing microbes mixture could decompose flee-straw with natural microorganism flora. |
| Keywords: | cellulose-decomposing bacteria mixed fermentation cellulase rice-straw |
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