灯盏花注射液抗新生鼠缺氧缺血脑损伤的药效作用

为观察灯盏花注射液治疗新生鼠缺氧缺血脑损伤的药物疗效及其药理作用机制,采用新生7日龄大鼠缺氧缺血性脑损伤模型,设立假手术组、缺氧缺血脑损伤模型组、灯盏花注射液治疗10,20,40 mg/kg剂量组、无菌注射用水对照组.采用硫堇染色、免疫组织化学染色的方法测定各实验组海马CA1区神经元密度、组织学分级、凋亡相关基因bcl-2和bax的表达情况,并计数各时间点Bcl-2和Bax蛋白免疫阳性细胞数目及积分光密度值.假手术组,大鼠海马CA1区无锥体细胞缺失,未见明显阳性细胞.与假手术组比较,缺氧缺血脑损伤模型组、无菌注射用水对照组Bcl-2,Bax蛋白表达均于3 d时达到高峰(与其余各时间点比较差别有显著意义p0.05),神经元密度明显降低,组织学分级显著增高,积分光密度值增加.灯盏花治疗组,与无菌注射用水对照组比较,Bcl-2阳性表达进一步增加,积分光密度值增加;而Bax阳性表达则减少,积分光密度值降低;神经元密度显著高于对照组,组织学分级明显降低.灯盏花注射液可能是通过上调Bcl-2表达,抑制Bax表达,减轻缺氧缺血引起的神经元凋亡及迟发性神经元死亡.

Curative Effect of Breviscapine Injection on the Hypoxic Ischemic Brain Damage of Neonatal Rats

To observe the curative effect of breviscapine injection on the hypoxic ischemic brain damage of neonatal rats and its mechanism,7-day-old newborn rats of hypoxic-ischemic brain damage modle were used and devided into four groups: sham group,model group,control with sterile water for injection group and breviscapine injection group.Breviscapine injection group was devied into 10,20 and 40 mg/kg doses.Using thionin staining and immunohistochemical wstaining the neuronal density,histological grade,and the expresssion of Bcl-2 and Bax protein in the CA1 hippocampus of each group,the number of positive cells and the integral optical density(IOD) of the immunostaining on Bcl-2,Bax protein expression in the CA1 hippocampus were examined.In sham group,there was no significant neuronal damage and no obvious positive cells of Bcl-2 and Bax in the CA1 hippocampus.In model group and controls with sterile water for injection group,the level of Bcl-2,Bax expression peaked at 3 d after hypoxic-ischemic brain damage(HIBD) (p<0.05 vs other groups),the value of neuronal density(ND) was decreased,and histological grade(HG) was increased compared with that in the sham group(p<0.05).Breviscapine injection group,compared with control with sterile water for injection group,the expression of Bcl-2 protein was further increased,IOD value increased,while the expression of Bax protein was decreased,IOD value decreased;The value of ND increased,and HG decreased.Breviscapin injection maybe could reduce the delayed neuronal death,and reduce the apoptosis of neuron after severe brain injury through improving the expression of Bcl-2 protein and inhibiting expression of Bax.The study will provide a fine theoretical foundation for clinical therapy of neonatal HIBD.