| 芸苔EPSPs基因cDNA的克隆和表达载体的构建 |
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| 引用本文： | 游大慧,骞宇,王健美,郭经宇,杨毅,李旭锋. 芸苔EPSPs基因cDNA的克隆和表达载体的构建[J]. 四川大学学报(自然科学版), 2004, 41(3): 661-664 |
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| 作者姓名： | 游大慧 骞宇 王健美 郭经宇 杨毅 李旭锋 |
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| 作者单位： | 四川大学生命科学学院,成都,610064;四川大学生命科学学院,成都,610064;四川大学生命科学学院,成都,610064;四川大学生命科学学院,成都,610064;四川大学生命科学学院,成都,610064;四川大学生命科学学院,成都,610064 |
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| 基金项目： | 四川省十五育种攻关项目 |
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| 摘 要： | 作者从芸苔(Brassica campestris)中用RT-PCR方法获得了EPSPs基因的cDNA．与其他物种中的EPSPs基因进行了比对和分析发现：芸苔EPSPs基因的cDNA与欧洲油菜的同源性最高,为93％,与水稻同源性最低,仅为64％．将芸苔EPSPs的ORF片段插入到GTK融合表达载体中,为EPSPs的原核表达奠定了基础。
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| 关 键 词： | EPSPs基因 芸苔 草甘膦 RT-PCR |
| 文章编号： | 0490-6756(2004)03-0661-04 |
The Cloning and Analysis of the cDNA of EPSPs Gene from Brassica campestris |
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| YOU Da-hui,QIAN Yu,WANG Jian-mei,GUO Jing-yu,YANG Yi,LI Xu-feng. The Cloning and Analysis of the cDNA of EPSPs Gene from Brassica campestris[J]. Journal of Sichuan University (Natural Science Edition), 2004, 41(3): 661-664 |
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| Authors: | YOU Da-hui QIAN Yu WANG Jian-mei GUO Jing-yu YANG Yi LI Xu-feng |
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| Abstract: | The cDNA of EPSPs is cloned and sequenced by RT-PCR from Brassica campestris.It is blasted with the EPSPs cDNA of other 8 organisms and it is found that the EPSPs cDNA from B.Campestris is most similar with that from B.napus, and least similar with that from O.sativa. The homology of EPSPs from B.Campestris and B.napus is 93%, and only 64% from B.Campestris and O.sativa. And also, the EPSPs cDNA is inserted into an expression vector,GTK, which is derived from the pGEX-2T, the product of Pharmacia. |
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| Keywords: | EPSPs gene Brassica campestris glyphosate RT-PCR |
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