Cloning, sequencing and expression of a swine IgG H chain gene inE. coli

A DNA fragment about 1.5 kb has been isolated from spleen of adult Chinese swine by RT-PCR. The DNA fragment encodes immunoglobulin IgG H chain gene. Sequencing analysis showed that the DNA fragment is 1 425 bp long, complete CDS. The C region of the gene has been classified as Subclass lg γ₃, and is the same as reported by Sunet al., but V region of the present gene is 42 bp less by comparison. The gene has been ligated into expression vector PET-3b (NSEB)(-). A protein about 52 ku has been expressed inE. coli with an expression level of about 21 %. The nucleotides sequence data reported in thin note have heen submitted to GenBank and assigned the accession numher ZHAOKAI AF062384. This note is a part of the firjt author’s Ph.D. dissenation.