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Coat protein promoter from cotton leaf curl virus is not a tissue-specifically expressed promoter
Authors:Yingqiu Xie  Zhen Zhu  Yule Liu  Qian Wu  Honglin Xu
Institution:(1) Institute of Genetics, Chinese Academy of Sciences, 100101 Beijing, China;(2) Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China
Abstract:Geminivirus is a kind of single-stranded DNA virus. Experimental results from tomato golden mosaic virus (TGMV) showed that expression pattern of coat protein gene (cp) promoter was phloem specifically expressed. In this note, the studies oncp promoter of cotton leaf curl virus (CLCuV) which is found and identified recently suggest that the promoter is not phloem specifically expressed. The expressing activity ofgus gene driven by the promoter exists not only in phloem but also in mesophyll tissues and root tip meristem. Transient expression suggests thatcp promoter transactivated by AC2 shows expressing activity in mesophyll and vascular tissue of leaf vein.
Keywords:CLCuV promoter  GUS  tobacco  cotton
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